CAJ | 학술논문

报道了一种新的检测黄曲霉毒素B1的生物传感器,该传感器以开管的多壁纳米碳管固定化黄曲霉毒素氧化还原酶制作传感电极检测黄曲霉毒素B1,其线性范围达到0.16μM-3.2μM,当把特异性的黄曲霉毒素B1抗体与黄曲霉毒素氧化还原酶通过多壁纳米碳管共固定化制作修饰电极,传感器的检测限提高到16nM,灵敏度提高了10倍.用这种方法制作黄曲霉毒素酶生物传感器,使黄曲霉毒素酶生物传感器向实用化迈进了一步.
보도료일충신적검측황곡매독소B1적생물전감기,해전감기이개관적다벽납미탄관고정화황곡매독소양화환원매제작전감전겁검측황곡매독소B1,기선성범위체도0.16μM-3.2μM,당파특이성적황곡매독소B1항체여황곡매독소양화환원매통과다벽납미탄관공고정화제작수식전겁,전감기적검측한제고도16nM,령민도제고료10배.용저충방법제작황곡매독소매생물전감기,사황곡매독소매생물전감기향실용화매진료일보.
A novel biosensor for aflatoxin B1 detecting has been reported. The biosensor electrode for AFB1 detecting was assembled by immobilized aflatoxin-oxidoreductase using open-ended multi-walled carbon nanotubes as matrix. Its linear range was between 0.16μM and 3.2μM. And if the specific anti-aflatoxin B1 antibody and aflatoxin oxidoreductase were both immobilized on the electrode with Multi-Walled carbon nanotubes, the detection limit of the modified electrode could be 16 nM with a 10 times improved sensitivity. The aflatoxin enzyme biosensor assembled this way strode one step forward its practical application.