CAJ | 학술논문

目的分离纯化苦瓜籽核糖体失活蛋白(RIP),对RIP在动物体内急性毒性和免疫毒性进行研究.方法采用离子交换层析、凝胶过滤层析等方法提取苦瓜籽RIP,对其进行小鼠急性毒性实验,确定小鼠半数致死量(LD_(50)),并通过豚鼠全身主动过敏试验和大鼠被动皮肤过敏试验,探讨苦瓜籽RIP的免疫毒性. 结果苦瓜籽RIP对小鼠的LD_(50)为25.2 mg/kg,全身主动过敏试验中,RIP高、低剂量组豚鼠出现明显过敏反应,多数迅速死亡,被动皮肤过敏试验中,RIP组大鼠皮肤出现明显蓝斑. 结论苦瓜籽核糖体失活蛋白具有较强的免疫毒性.
목적 분리순화고과자핵당체실활단백(RIP),대RIP재동물체내급성독성화면역독성진행연구.방법 채용리자교환층석、응효과려층석등방법제취고과자RIP,대기진행소서급성독성실험,학정소서반수치사량(LD_(50)),병통과돈서전신주동과민시험화대서피동피부과민시험,탐토고과자RIP적면역독성. 결과고과자RIP대소서적LD_(50)위25.2 mg/kg,전신주동과민시험중,RIP고、저제량조돈서출현명현과민반응,다수신속사망,피동피부과민시험중,RIP조대서피부출현명현람반. 결론 고과자핵당체실활단백구유교강적면역독성.
Objective To separate and purify ribosome inhibiting protein (RIP) from Momordica charantia (bitter melon) seeds and to evaluate its acute toxicity and immunotoxicity in animal. Methods Ion exchange chromatography and gel filtration chromatography were applied in the separating and purifying of RIP from Momordica charantia seeds. Then the acute toxicity testing of RIP in mice was conducted to obtain its half lethal dose (LD_(50)). Active systemic anaphylaxis (ASA) test in guinea pig and passive cutaneous anaphylaxis test (PCA) in rat were performed to evaluate its immunotoxicity. Results The LD_(50) (iv) in mice of RIP was 25. 2 mg/kg in ASA. guinea pigs of the higher and lower RIP group all appeared stong allergic responses and most of them died quickly. In PCA. obvious blue dye in skin were observed in SD rats of the RIP group. Conclusion RIP getting from Momordica charantia seeds had a relatively strong immunotoxicity in animals.