CAJ | 학술논문

目的通过比较分析葡萄糖-6-磷酸异构酶(G6PT)mRNA在类风湿关节炎(RA)、其他自身免疫性疾病及健康人群之间的表达差异,探讨其在RA中的致病机制.方法建立实时定量RT-PCR方法,并利用该法检测128例RA患者(RA组)、32例其他自身免疫件疾病患者(疾病对照组)、100例健康体检者(健康对照组)G6PI基因的表达水平.结果经GAPDH校正后G6PI基因表达水平在RA组为2.72±2.09,其中活动组和缓解组分别为3.12±2.40和2.13±1.34,疾病对照组为2.14±1.43,健康对照组为1.25±0.73.RA组显著高于健康对照组(P<0.01),RA活动组基因表达水平显著高于缓解组(P<0.01),也显著高于疾病对照组(P<0.01).结论 G6PI在RA患者中表达的显著增加表明G6PI可能参与了RA的发病过程,并与疾病的活动性相关,可作为RA的辅助件诊断指标及早期筛查指标.
목적 통과비교분석포도당-6-린산이구매(G6PT)mRNA재류풍습관절염(RA)、기타자신면역성질병급건강인군지간적표체차이,탐토기재RA중적치병궤제.방법 건립실시정량RT-PCR방법,병이용해법검측128례RA환자(RA조)、32례기타자신면역건질병환자(질병대조조)、100례건강체검자(건강대조조)G6PI기인적표체수평.결과 경GAPDH교정후G6PI기인표체수평재RA조위2.72±2.09,기중활동조화완해조분별위3.12±2.40화2.13±1.34,질병대조조위2.14±1.43,건강대조조위1.25±0.73.RA조현저고우건강대조조(P<0.01),RA활동조기인표체수평현저고우완해조(P<0.01),야현저고우질병대조조(P<0.01).결론 G6PI재RA환자중표체적현저증가표명G6PI가능삼여료RA적발병과정,병여질병적활동성상관,가작위RA적보조건진단지표급조기사사지표.
Objective To investigate the expression of mRNA of the glucose-6-phosphate isomerase(G6PI) in rheumatoid ar-thritis(RA) ,non-RA patients and healthy controls to preliminarily study the pathogenic mechanism of RA. Methods A real-time quantitative reverse transcriptase polymerase chain reaction(RQ RT-PCR) method was established for detecting G6PI and internal reference GAPDH expression levels in 128 RA patients,32 non-RA patients and 100 healthy controls. Results The level of G6PI mRNA in RA patients 2.72±22.09 was higher than that of the healthy controls(1.25±0.73) (P<0.01). In RA patients,the ex-pression level of G6PI mRNA in the active stage(3.12±2.40) was higher than that at the stable stage(2. 13±1.34)(P<0. 01). Conclusion The increased expression of G6PI may play a pathological role in the development of RA,and it may be correlated with the activity of RA. G6PI, which can be regarded as a marker for early detection of RA.