CAJ | 학술논문

本研究旨在探讨高铜对雏鸡脑组织抗氧化酶活性的影响.将360羽1日龄艾维茵肉鸡健雏随机分为6组,分别喂以对照日粮(10.89 mg·kg~(-1))和高铜日粮(Cu 100 mg·kg~(-1),高铜Ⅰ组;Cu 200 mg·kg~(-1),高铜Ⅱ组;Cu 400 mg·kg~(-1),高铜Ⅲ组;Cu 600 mg·kg~(-1),高铜Ⅳ组;Cu 800 mg·kg~(-1),高铜Ⅴ组)6周.试验第14、28、42天每组随机抽取5羽鸡剖杀后,测定脑组织胆碱酯酶(CHE)、单胺氧化酶(MAO)、过氧化氢酶(CAT)、铜锌超氧化物歧化酶(Cu-Zn-SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性.脑组织CHE和CAT活性随日粮铜含量的升高而降低,高铜Ⅱ、Ⅲ、Ⅳ和Ⅴ组与对照组比较差异极显著(P<0.01);脑组织MAO活性随日粮铜含量的升高而升高,高铜Ⅱ、Ⅲ、Ⅳ和Ⅴ组与对照组比较差异显著或极显著(P<0.05或P<0.01);脑组织Cu-Zn-SOD和GSH-Px活性,高铜Ⅰ、Ⅱ组显著或极显著高于对照组(P<0.05或P<0.01),高铜Ⅲ、Ⅳ和Ⅴ组极显著降低(P<0.01).日粮铜水平在400～800 mg·kg~(-1)时,脑组织的抗氧化酶活性降低,脑组织的抗氧化功能下降.
본연구지재탐토고동대추계뇌조직항양화매활성적영향.장360우1일령애유인육계건추수궤분위6조,분별위이대조일량(10.89 mg·kg~(-1))화고동일량(Cu 100 mg·kg~(-1),고동Ⅰ조;Cu 200 mg·kg~(-1),고동Ⅱ조;Cu 400 mg·kg~(-1),고동Ⅲ조;Cu 600 mg·kg~(-1),고동Ⅳ조;Cu 800 mg·kg~(-1),고동Ⅴ조)6주.시험제14、28、42천매조수궤추취5우계부살후,측정뇌조직담감지매(CHE)、단알양화매(MAO)、과양화경매(CAT)、동자초양화물기화매(Cu-Zn-SOD)화곡광감태과양화물매(GSH-Px)활성.뇌조직CHE화CAT활성수일량동함량적승고이강저,고동Ⅱ、Ⅲ、Ⅳ화Ⅴ조여대조조비교차이겁현저(P<0.01);뇌조직MAO활성수일량동함량적승고이승고,고동Ⅱ、Ⅲ、Ⅳ화Ⅴ조여대조조비교차이현저혹겁현저(P<0.05혹P<0.01);뇌조직Cu-Zn-SOD화GSH-Px활성,고동Ⅰ、Ⅱ조현저혹겁현저고우대조조(P<0.05혹P<0.01),고동Ⅲ、Ⅳ화Ⅴ조겁현저강저(P<0.01).일량동수평재400～800 mg·kg~(-1)시,뇌조직적항양화매활성강저,뇌조직적항양화공능하강.
The experiment was conducted to examine the effect of dietary high copper on the antioxidase activities of brain tissue in chickens. 360 one-day-old Avian chickens were randomly divided into six groups and fed with the diets supplemented with 10.89 (control),100 (high copper group Ⅰ),200(high copper group Ⅱ),400 (high copper group Ⅲ), 600 (high copper group Ⅳ), and 800(high copper group Ⅴ) mg Cu per kilogram for six weeks, respectively. 5 chickens of each group were killed and their brain samples were collected at the 14, 28, 42 days. The activities of cholinesterase(CHE), catalase (CAT), monoamine oxidase (MAO), cuprozinc-super-oxide dismutase(Cu-Zn-SOD) and glutathione peroxidase (GSH-Px) were detected. Compared with the control group, the activities of CHE and CAT were significantly lower(P<0.01) and the activity of MAO was significantly higher(P<0.01 or P<0.05) in high copper groups Ⅱ, Ⅲ,Ⅳ and Ⅴ. The activities of Cu-Zn-SOD and GSH-Px were lower in high copper groups Ⅲ, Ⅳ and V than those in control group(P<0.01). The results showed that 400-800 mg·kg~(-1)dietary copper could depress the activities of antioxidase in brain tissue, and the antioxygen function of brain tissue was decreased.