CAJ | 학술논문

[目的]筛选能高效降解三唑磷的菌株,并研究其降解特性.[方法]从三唑磷生产厂的曝气池活性污泥中分离到1株三唑磷降解菌C-Y106,并根据C-Y106的形态、生理生化特征和16S rRNA同源性序列分析进行了菌株鉴定,同时研究了不同营养的培养基对C-Y106降解三唑磷速率的影响.[结果]经鉴定,C-Y106为枯草芽孢杆菌.C-Y106能以三唑磷为唯一碳源、唯一氮源、唯一磷源生长,其中以三唑磷为唯一磷源时其降解速率最大,且在31 ℃、初始pH 8.0、150 r/min条件下,培养60 h后,三唑磷降解率为76.8%.[结论]该研究为三唑磷降解酶的分离纯化提供了理论依据.
[목적]사선능고효강해삼서린적균주,병연구기강해특성.[방법]종삼서린생산엄적폭기지활성오니중분리도1주삼서린강해균C-Y106,병근거C-Y106적형태、생리생화특정화16S rRNA동원성서렬분석진행료균주감정,동시연구료불동영양적배양기대C-Y106강해삼서린속솔적영향.[결과]경감정,C-Y106위고초아포간균.C-Y106능이삼서린위유일탄원、유일담원、유일린원생장,기중이삼서린위유일린원시기강해속솔최대,차재31 ℃、초시pH 8.0、150 r/min조건하,배양60 h후,삼서린강해솔위76.8%.[결론]해연구위삼서린강해매적분리순화제공료이론의거.
[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of triazophos manufacture. Then the strain C-Y106 was identified according to the morphology,physiological and biochemical characteristics,and 16S rRNA sequence analysis. The effect of medium with different nutrients on triazophos-degrading rate by C-Y106 was studied. [Result] The strain C-Y106 was identified as Bacillus subtilis. The strain C-Y106 could grow in the mineral salt medium with 40 mg/L of triazophos as the sole sources of carbon,Nitrogen and Phosphorus. The triazophos-degrading rate was the highest as 76.8% in the mineral salt medium with 40 mg/L of triazophos as the sole source of Phosphorus,after being incubated at 31 ℃,pH 8.0 and 150 r/min for 60 h. [Conclusion] The research had provided theoretical basis for the identification and purification of enzymes for triazophos degradation.