CAJ | 학술논문

[目的]探讨水稻应答N+束辐照机制中相关miRNA 的表达差异.[方法]各选取3组N+束辐照处理水稻种子和未处理的水稻种子,提取其在30 ℃发芽96 h幼苗总RNA,采用Agilent单通道水稻基因表达谱芯片筛选2组水稻幼苗差异表达基因.芯片中含有miRNA 探针510个,分析其在样品间表达差异2 倍左右作为域值范围.[结果]辐照组与对照组相比表达差异显著的miRNA分子有14个,且均为下调.[结论]水稻N+束辐照组和对照组中存在差异表达的miRNA分子,可能调控某些基因的表达来应答离子辐照,从而表现出与对照不一样的生理生化和表型差异.
[목적]탐토수도응답N+속복조궤제중상관miRNA 적표체차이.[방법]각선취3조N+속복조처리수도충자화미처리적수도충자,제취기재30 ℃발아96 h유묘총RNA,채용Agilent단통도수도기인표체보심편사선2조수도유묘차이표체기인.심편중함유miRNA 탐침510개,분석기재양품간표체차이2 배좌우작위역치범위.[결과]복조조여대조조상비표체차이현저적miRNA분자유14개,차균위하조.[결론]수도N+속복조조화대조조중존재차이표체적miRNA분자,가능조공모사기인적표체래응답리자복조,종이표현출여대조불일양적생리생화화표형차이.
[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N+ beam radiation in rice. [Method] Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively, and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃. Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings. The chip contained 510 miRNA probes; and about two times of expression differences between two samples were considered as the threshold range. [Result] 14 miRNA molecules showed significant expression differences between the irradiated group and the control group, and all of them were decreased. [Conclusion] The miRNAs showed expression differences between irradiated group and the control group, which might regulate the expression of certain genes to respond to ion irradiation, thus producing physiological, biochemical and phenotypic differences.