中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2001年
1期
27-30
,共4页
癌,肝细胞%CD34%新生血管化%免疫组织化学
癌,肝細胞%CD34%新生血管化%免疫組織化學
암,간세포%CD34%신생혈관화%면역조직화학
目的 探讨原发性肝细胞癌（HCC）新生血管生成与临床病理指标的关系及其可否用于预测HCC侵袭、转移和预后。方法 采用免疫组织化学方法，检测了20例正常肝组织、20例肝硬化组织、85例HCC及癌旁组织中CD34的表达，以及HCC的微血管密度（MVD）。结果 CD34的染色定位在血管内皮细胞上，HCC窦样血管CD34表达为强阳性；除门管区小血管分支及中央静脉外，癌旁肝组织、肝硬化组织、正常肝组织基本不表达CD34；HCC的MVD范围（18～360）/0.74 mm2、（156.5±62.4）/0.74 mm2。HCC的MVD与肿瘤大小、病灶数目、分化程度、门静脉瘤栓形成、包膜状况均有显著关系（P＜0.05或0.01）；与HBsAg是否阳性、术前AFP水平无关（P＞0.05）；与预后亦有关，少血管组（MVD＜156）预后显著优于多血管组（MVD≥156），术后平均无瘤生存期少血管组为53个月，多血管组为14个月，（P＜0.001）。结论 CD34的表达反映了HCC的新生血管化，与HCC的发展有关。MVD可作为判断HCC侵袭、转移和预后的指标。
目的 探討原髮性肝細胞癌（HCC）新生血管生成與臨床病理指標的關繫及其可否用于預測HCC侵襲、轉移和預後。方法 採用免疫組織化學方法，檢測瞭20例正常肝組織、20例肝硬化組織、85例HCC及癌徬組織中CD34的錶達，以及HCC的微血管密度（MVD）。結果 CD34的染色定位在血管內皮細胞上，HCC竇樣血管CD34錶達為彊暘性；除門管區小血管分支及中央靜脈外，癌徬肝組織、肝硬化組織、正常肝組織基本不錶達CD34；HCC的MVD範圍（18～360）/0.74 mm2、（156.5±62.4）/0.74 mm2。HCC的MVD與腫瘤大小、病竈數目、分化程度、門靜脈瘤栓形成、包膜狀況均有顯著關繫（P＜0.05或0.01）；與HBsAg是否暘性、術前AFP水平無關（P＞0.05）；與預後亦有關，少血管組（MVD＜156）預後顯著優于多血管組（MVD≥156），術後平均無瘤生存期少血管組為53箇月，多血管組為14箇月，（P＜0.001）。結論 CD34的錶達反映瞭HCC的新生血管化，與HCC的髮展有關。MVD可作為判斷HCC侵襲、轉移和預後的指標。
목적 탐토원발성간세포암（HCC）신생혈관생성여림상병리지표적관계급기가부용우예측HCC침습、전이화예후。방법 채용면역조직화학방법，검측료20례정상간조직、20례간경화조직、85례HCC급암방조직중CD34적표체，이급HCC적미혈관밀도（MVD）。결과 CD34적염색정위재혈관내피세포상，HCC두양혈관CD34표체위강양성；제문관구소혈관분지급중앙정맥외，암방간조직、간경화조직、정상간조직기본불표체CD34；HCC적MVD범위（18～360）/0.74 mm2、（156.5±62.4）/0.74 mm2。HCC적MVD여종류대소、병조수목、분화정도、문정맥류전형성、포막상황균유현저관계（P＜0.05혹0.01）；여HBsAg시부양성、술전AFP수평무관（P＞0.05）；여예후역유관，소혈관조（MVD＜156）예후현저우우다혈관조（MVD≥156），술후평균무류생존기소혈관조위53개월，다혈관조위14개월，（P＜0.001）。결론 CD34적표체반영료HCC적신생혈관화，여HCC적발전유관。MVD가작위판단HCC침습、전이화예후적지표。
Objective To investigate the relationship between the tumor angiogenesis and the clinicopathological variables of hepatocellular carcinoma (HCC) and determine whether tumor angiogenesis can be used as an indicator for predicting invasion, metastasis and prognosis of HCC. Methods Using the immunohistochemical S-P method, CD34 expression was detected in liver tissue from 20 normal individuals, 20 patients with liver cirrhosis and 85 patients with HCC and juxtacancerous tissues and the microvascular density (MVD) determined in those from 85 patients with HCC. Results CD34 was mainly located in the cell cytoplasm of endothelial cells. The CD34 staining was confined to vessels in the portal triad in the above-mentioned various tissues. In HCC tissues, however, sinusoid-like vessels reacted intensively with anti-CD34 and the MVD was 156.5±62.4 (per 0.74 mm2). MVD was correlated to tumor size, number, differentiation, emboli in the portal vein and capsule (P<0.05 or 0.01) but not to HBsAg and AFP (P>0.05). It was also correlated with prognosis of HCC. The mean tumor-free survival of MVD ≥156 was 14 months while that of MVD <156 was 53 months (P<0.01). Conclusion The expression of CD34 in sinusoid-like vessels in HCC tissues represents the neovascularization. Angiogenesis in HCC is associated with the progression of HCC. MVD can be used as an indicator to judge the invasion, metastasis and prognosis of HCC.
