CAJ | 학술논문

目的研究弓形虫感染后雄性大鼠Th1、Th2细胞因子IFN-γ、IL-4及一氧化氮(NO)水平的变化,探讨其对雄性睾丸生精细胞损伤的影响.方法选用9～10周龄雄性SD大鼠132只,按随机数字表法分为3组:正常对照组、低剂量感染组(1×102个速殖子)、高剂量感染组(1×104个速殖子),感染后第0、3、6、9、……30天,感染组和对照组分别取4只大鼠,检测大鼠血清IFN-γ、IL-4及NO的水平,并且做睾丸的病理学检查和精子计数.结果弓形虫感染组大鼠血清IFN-γ显著升高,低剂量组及高剂量组在第6天达峰值,分别为(286.3±45.3) pg/ml和(506.6±34.3) pg/ml；血清NO亦显著升高,高剂量组第9天达峰值(77.7±7.0) μmol/L,低剂量组第12天达峰值(59.5±5.3) μmol/L,之后均迅速降低；感染组大鼠血清IL-4逐渐升高,低剂量组及高剂量组于第15天达峰值(233.3±36.9) pg/ml、(366.7±52.4) pg/ml后逐渐降低.以上指标至实验末期均未恢复正常水平.病理检查显示,正常对照组大鼠生精小管层次清晰,细胞丰富,精子计数为175.7±23.7,感染组大鼠生精小管层次混乱,精母细胞、精子细胞明显减少,低剂量组及高剂量组至第15天达谷值84.5.23.5、47.3±14.7,至实验末期无明显恢复.结论弓形虫感染导致的早期Th1细胞因子IFN-γ极化及NO显著升高,随后引起Th2细胞因子IL-4反应性增高,并介导以精母细胞为主的生精细胞损伤及生精障碍.
목적 연구궁형충감염후웅성대서Th1、Th2세포인자IFN-γ、IL-4급일양화담(NO)수평적변화,탐토기대웅성고환생정세포손상적영향.방법 선용9～10주령웅성SD대서132지,안수궤수자표법분위3조:정상대조조、저제량감염조(1×102개속식자)、고제량감염조(1×104개속식자),감염후제0、3、6、9、……30천,감염조화대조조분별취4지대서,검측대서혈청IFN-γ、IL-4급NO적수평,병차주고환적병이학검사화정자계수.결과 궁형충감염조대서혈청IFN-γ현저승고,저제량조급고제량조재제6천체봉치,분별위(286.3±45.3) pg/ml화(506.6±34.3) pg/ml；혈청NO역현저승고,고제량조제9천체봉치(77.7±7.0) μmol/L,저제량조제12천체봉치(59.5±5.3) μmol/L,지후균신속강저；감염조대서혈청IL-4축점승고,저제량조급고제량조우제15천체봉치(233.3±36.9) pg/ml、(366.7±52.4) pg/ml후축점강저.이상지표지실험말기균미회복정상수평.병리검사현시,정상대조조대서생정소관층차청석,세포봉부,정자계수위175.7±23.7,감염조대서생정소관층차혼란,정모세포、정자세포명현감소,저제량조급고제량조지제15천체곡치84.5.23.5、47.3±14.7,지실험말기무명현회복.결론 궁형충감염도치적조기Th1세포인자IFN-γ겁화급NO현저승고,수후인기Th2세포인자IL-4반응성증고,병개도이정모세포위주적생정세포손상급생정장애.
Objective To study the effect of fluctuation of Th1,Th2 cytokines IFN-γ,IL-4 and nitric oxide on spermatogenic cells in male rats after Toxoplasma infection.Methods 132 male SD-rats(9-10 week)were randomly divided into three groups:negative control group,low dose infection group( 1 × l02 tachyzoites)and high dose infection group ( 1 × 104 tachyzoites ). Four rats of control group and infection groups were excuted on the 0,3,6,9...and 30 day after infection,then the levels of serum IFN-γ,IL-4,NO were detected,the pathology of spermatogenic cells examined and sperms counted. Results IFN-γ in serum increased significantly in infection groups,low dose infection group and high dose infection group reaching the peak on 6 d,respectively (286.3 ±45.3) pg/ml and (506.6 ±34.3) pg/ml ; NO in serum increased significantly too,high dose group on 9 d(77.7 ± 7.0)μmol/L while low dose group got to peak on 12 d( 59.5 ± 5.3 ) μmol/L,then both decreased rapidly.IL-4 in serum of infection groups rats increased gradully,low dose group(233.3 ±36.9) pg/ml and high dose group(366.7 ±52.4) pg/ml reaching the peak on 15 d and then deceased.However,the levels of above cytokines did not return to normal till the termination of experiment.Pathological examination showed clear seminiferous tubules layers and rich cells in nomal control group rats with sperm counted 175.7 + 23.7,while in infection group rats' seminiferous tubules layers were in disorder and spermatocytes,spermatids reduced significantly,which counted as low dose group ( 84.5 + 23.5) and high dose group (47.3 +14.7) to valley value on 15 d.Those changes did not recover at the end of the experiment.Conclusion Toxoplasma gondii infection led to Th1 cytokines IFN-γ polarization and NO increasing in early stage,followed by Th2 cytokine IL-4 increasing reactively.As a result,spermatogenic cells mainly spermatocytes were damaged and spermatogenesis impaired.