CAJ | 학술논문

目的研究日本血吸虫DNA鸡尾酒疫苗的免疫保护效果. 方法制备日本血吸虫谷胱甘肽S-转移酶(glutathione S-transferase,GST)、硫氧还蛋白(thioredoxins, Trx)、次黄嘌呤鸟嘌呤磷酸核糖转移酶(hypoxanthine-guanine phosphoriboribosyl-transferase,HGPRT)DNA疫苗,免疫C57BL/6小鼠.C57BE/6小鼠随机分为感染对照组、空质粒对照组、pVAX1-GST免疫组、pVAX1-Trx免疫组、pVAX1-HGPRT免疫组及DNA鸡尾酒疫苗组,依次注射生理盐水,空质粒pVAX1,pVAX1-GST,pVAX1-Trx,pVAX1-HGPRT和由pVAX1-GST、pVAX1-Trx、pVAX1-HGPRT等3种质粒混合而成的DNA鸡尾酒疫苗.每组小鼠于0、2、4周经股四头肌注射免疫,每次间隔2周.末次免疫后2周,每只小鼠经腹部皮肤感染30±1条日本血吸虫尾蚴.感染后42 d剖杀小鼠,并计数各组小鼠血吸虫成虫数和肝虫卵数. 结果 pVAX1-GST、pVAX1-Trx、pVAX1-HGPRT和DNA鸡尾酒疫苗免疫组小鼠的减虫率依次为0、35.78%、47.89%和48.13%,肝减卵率依次为22.94%、34.14%、43.35%和26.77%. 结论 pVAX1-Trx、pVAX1-HGPRT及DNA鸡尾酒疫苗具有一定的抗日本血吸虫病作用,但3种疫苗联合使用并未发现有显著的协同作用.
목적 연구일본혈흡충DNA계미주역묘적면역보호효과. 방법 제비일본혈흡충곡광감태S-전이매(glutathione S-transferase,GST)、류양환단백(thioredoxins, Trx)、차황표령조표령린산핵당전이매(hypoxanthine-guanine phosphoriboribosyl-transferase,HGPRT)DNA역묘,면역C57BL/6소서.C57BE/6소서수궤분위감염대조조、공질립대조조、pVAX1-GST면역조、pVAX1-Trx면역조、pVAX1-HGPRT면역조급DNA계미주역묘조,의차주사생리염수,공질립pVAX1,pVAX1-GST,pVAX1-Trx,pVAX1-HGPRT화유pVAX1-GST、pVAX1-Trx、pVAX1-HGPRT등3충질립혼합이성적DNA계미주역묘.매조소서우0、2、4주경고사두기주사면역,매차간격2주.말차면역후2주,매지소서경복부피부감염30±1조일본혈흡충미유.감염후42 d부살소서,병계수각조소서혈흡충성충수화간충란수. 결과 pVAX1-GST、pVAX1-Trx、pVAX1-HGPRT화DNA계미주역묘면역조소서적감충솔의차위0、35.78%、47.89%화48.13%,간감란솔의차위22.94%、34.14%、43.35%화26.77%. 결론 pVAX1-Trx、pVAX1-HGPRT급DNA계미주역묘구유일정적항일본혈흡충병작용,단3충역묘연합사용병미발현유현저적협동작용.
Objective To investigate the protective immune efficacy against Schistosoma japonicum infection induced by cocktail DNA vaccines. Methods The male C57BL/6 mice were randomly divided into 6 groups (infected control group, pVAX1 group, pVAX1-GST group, pVAX1-Trx group, pVAX1-HGPRT group, and cocktail DNA vaccine group). The mice in each group were injected with saline, plasmid pVAX1, pVAX1-GST, pVAX1-Trx, pVAX1-HGPRT, and the cocktail vaccine (mixture of pVAX1-GST, pVAX1-Trx and pVAX1-HGPRT) respectively for three times at 0, 2 and 4 week with interval of two weeks. Two weeks after the last immunization, all mice were challenged with cercariae of S. japonicum. Forty-two days post-challenge infection, the mice were sacrificed. The numbers of recovered worms and eggs in liver were counted. Result The worm reduction rates in pVAX1-GST group, pVAX1-Trx group, pVAX1-HGPRT group and cocktail DNA vaccine group were 0, 35.78% , 47.89% and 48. 13% respectively, while the egg reduction rates were 22.94% ,34. 14% ,43. 35% and 26. 77% , respectively. Conclusion The pVAX1-Trx, pVAX1-HGPRT and cocktail DNA vaccine exhibited a certain level of protective efficacy against schistosomiasis, however there were no significant synergetic effects using the cocktail DNA vaccine.