中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2010年
2期
96-100
,共5页
肝炎病毒,乙型%颗粒酶类%干扰素γ%肿瘤坏死因子α%穿孔素%颗粒溶素
肝炎病毒,乙型%顆粒酶類%榦擾素γ%腫瘤壞死因子α%穿孔素%顆粒溶素
간염병독,을형%과립매류%간우소γ%종류배사인자α%천공소%과립용소
Hepatitis B virus%Granzymes%Interferon gamma%Tumor necrosis factor alpha%Perforin%Granulysin
目的 研究慢性HBV感染者外周血自然杀伤细胞(NK细胞)免疫效应分子表达与感染状态的相关性.方法 健康人作为对照组,慢性HBV感染者根据感染状态分为肝功正常高病毒复制组、肝功能正常低病毒复制组、肝功能异常高病毒复制组、肝功能异常低病毒复制组.应用流式细胞法检测外周血NK细胞的穿孔素(PF)、颗粒酶B(Gr B)、颗粒溶素(GNLY)、肿瘤坏死因子(TNF)α和干扰素(IFN)γ的表达,分析其与感染状态的相关性.组间比较用单因素方差分析;相关性分析采用直线相关Pearson分析.结果 阳性细胞百分比同对照组(31.50%±27.64%)相比,肝功能正常高病毒复制组(59.74%±30.82%)和肝功能正常低病毒复制组(61.89%±33.30%)的GNLY表达增加有统计学意义;同对照组(57.38%±23.69%)相比,肝功正常高病毒复制组(39.89%±21.30%)、肝功能异常高病毒复制组(37.54%±18.79%)的IFN γ表达降低,P值均<0.05,差异有统计学意义;肝功能正常高病毒复制组的PF、Gr B、GNLY(阳性细胞百分比分别为56.98%±38.34%、81.53%±19.58%、59.74%±30.82%)和肝功能正常低病毒复制组的PF、Gr B、GNLY(阳性细胞百分比分别为62.95%±31.98%、84.51%±14.57%、61.89%±33.30%)表达高于肝功能异常高病毒复制组 (阳性细胞百分比分别为35.47%±29.64%,66.55%±22.92%,42.03±33.17%),P值均<0.05,差异有统计学意义;除了TNF α和GNLY不具有相关性外,PF、Gr B、GNLY、TNF α和IFN γ之间呈两两正相关关系.结论 高病毒复制水平的HBV感染者外周血NK细胞表达的IFN γ明显低于正常对照组;肝功能正常的HBV感染者外周血NK细胞表达的PF、Gr B、GNLY水平明显高于肝功能异常者.
目的 研究慢性HBV感染者外週血自然殺傷細胞(NK細胞)免疫效應分子錶達與感染狀態的相關性.方法 健康人作為對照組,慢性HBV感染者根據感染狀態分為肝功正常高病毒複製組、肝功能正常低病毒複製組、肝功能異常高病毒複製組、肝功能異常低病毒複製組.應用流式細胞法檢測外週血NK細胞的穿孔素(PF)、顆粒酶B(Gr B)、顆粒溶素(GNLY)、腫瘤壞死因子(TNF)α和榦擾素(IFN)γ的錶達,分析其與感染狀態的相關性.組間比較用單因素方差分析;相關性分析採用直線相關Pearson分析.結果 暘性細胞百分比同對照組(31.50%±27.64%)相比,肝功能正常高病毒複製組(59.74%±30.82%)和肝功能正常低病毒複製組(61.89%±33.30%)的GNLY錶達增加有統計學意義;同對照組(57.38%±23.69%)相比,肝功正常高病毒複製組(39.89%±21.30%)、肝功能異常高病毒複製組(37.54%±18.79%)的IFN γ錶達降低,P值均<0.05,差異有統計學意義;肝功能正常高病毒複製組的PF、Gr B、GNLY(暘性細胞百分比分彆為56.98%±38.34%、81.53%±19.58%、59.74%±30.82%)和肝功能正常低病毒複製組的PF、Gr B、GNLY(暘性細胞百分比分彆為62.95%±31.98%、84.51%±14.57%、61.89%±33.30%)錶達高于肝功能異常高病毒複製組 (暘性細胞百分比分彆為35.47%±29.64%,66.55%±22.92%,42.03±33.17%),P值均<0.05,差異有統計學意義;除瞭TNF α和GNLY不具有相關性外,PF、Gr B、GNLY、TNF α和IFN γ之間呈兩兩正相關關繫.結論 高病毒複製水平的HBV感染者外週血NK細胞錶達的IFN γ明顯低于正常對照組;肝功能正常的HBV感染者外週血NK細胞錶達的PF、Gr B、GNLY水平明顯高于肝功能異常者.
목적 연구만성HBV감염자외주혈자연살상세포(NK세포)면역효응분자표체여감염상태적상관성.방법 건강인작위대조조,만성HBV감염자근거감염상태분위간공정상고병독복제조、간공능정상저병독복제조、간공능이상고병독복제조、간공능이상저병독복제조.응용류식세포법검측외주혈NK세포적천공소(PF)、과립매B(Gr B)、과립용소(GNLY)、종류배사인자(TNF)α화간우소(IFN)γ적표체,분석기여감염상태적상관성.조간비교용단인소방차분석;상관성분석채용직선상관Pearson분석.결과 양성세포백분비동대조조(31.50%±27.64%)상비,간공능정상고병독복제조(59.74%±30.82%)화간공능정상저병독복제조(61.89%±33.30%)적GNLY표체증가유통계학의의;동대조조(57.38%±23.69%)상비,간공정상고병독복제조(39.89%±21.30%)、간공능이상고병독복제조(37.54%±18.79%)적IFN γ표체강저,P치균<0.05,차이유통계학의의;간공능정상고병독복제조적PF、Gr B、GNLY(양성세포백분비분별위56.98%±38.34%、81.53%±19.58%、59.74%±30.82%)화간공능정상저병독복제조적PF、Gr B、GNLY(양성세포백분비분별위62.95%±31.98%、84.51%±14.57%、61.89%±33.30%)표체고우간공능이상고병독복제조 (양성세포백분비분별위35.47%±29.64%,66.55%±22.92%,42.03±33.17%),P치균<0.05,차이유통계학의의;제료TNF α화GNLY불구유상관성외,PF、Gr B、GNLY、TNF α화IFN γ지간정량량정상관관계.결론 고병독복제수평적HBV감염자외주혈NK세포표체적IFN γ명현저우정상대조조;간공능정상적HBV감염자외주혈NK세포표체적PF、Gr B、GNLY수평명현고우간공능이상자.
Objective To investigate the expression profile of immune effector molecules in periph-eral natural killer cells (NK) in patients with chronic hepatitis virus B. Methods According to the infection status, patients were divided into four experiment groups: normal hepatic function and high HBV DNA level group, normal hepatic function and low HBV DNA level group, abnormal hepatic function and high HBV DNA level group and abnormal hepatic function and low HBV DNA level group. The expression of perforin (PF), granzyme B (Gr B), granulysin (GNLY), tumor necrosis factor alpha (TNF α ) and interferon gamma (IFN γ ) in NK cells were detected by flow cytometer. Results Compared with control group (31.50% ± 27.64%), the expression of GNLY was significantly increased in normal hepatic function and high HBV DNA level group (59.74% ± 30.82%) and normal hepatic function and low HBV DNA level group (61.89% ± 33.30%);the expression of IFN γ in normal hepatic function and high HBV DNA level group (39.89% ± 21.30%) and abnormal hepatic function and high HBV DNA level group (37.54% ± 18.79%) was lower than that in normal control group (57.38% ± 23.69%);the expression of PF, GrB, GNLY in abnormal hepatic function and high HBV DNA level group (35.47% ± 29.64%, 66.55% ± 22.92%, 42.03% ± 33.17%) was lower than that in normal hepatic function and high HBV DNA level group (56.98% ± 38.34%, 81.53% ± 19.58%, 59.74% ± 30.82%) and normal hepatic function and low HBV DNA level groups (62.95% ± 31.98%, 84.51% ± 14.57%, 61.89% ± 33.3%);there were positive correlations between ef PF, Gr B, GNLY, TNF α, and IFN γ. Conclusion The expression of IFN γ in NK cells from patients with high HBV DNA replication level is lower than that in normal control group;the expression of PF, Gr B and GNLY in NK cells from patients with normal hepatic function is higher than that in NK cells from patients with abnormal hepatic function.
