中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2010年
7期
733-736
,共4页
周红丽%韩亚荣%靳蕊霞%黄波
週紅麗%韓亞榮%靳蕊霞%黃波
주홍려%한아영%근예하%황파
叔丁基过氧化氢%鼠肾小球系膜细胞%衰老%普罗布考
叔丁基過氧化氫%鼠腎小毬繫膜細胞%衰老%普囉佈攷
숙정기과양화경%서신소구계막세포%쇠로%보라포고
Tert-Butyl hydroperoxide%Human glomerular mesangial cells%Cell senescence%Probucol
目的 探讨叔丁基过氧化氢体外诱导鼠肾小球系膜细胞衰老及普罗布考延缓衰老的作用.方法 应用不同浓度叔丁基过氧化氢刺激鼠系膜细胞,每天刺激1 h,连续作用4 d.刺激结束后48 h检测细胞存活率、β-半乳糖苷酶染色阳性细胞率、细胞周期情况鉴定衰老细胞,并透射电镜观察衰老细胞超微结构,并观察普罗布考对上述指标的影响.结果 30μmol/L叔丁基过氧化氢诱导组细胞存活率为对照组的(80.12±3.25)%(P<0.05),约81%的细胞呈现β-半乳糖苷酶染色阳性,流式细胞仪检测86%细胞阻滞于G0、G1期,透射电镜可见叔丁基过氧化氢诱导组细胞核膜内陷,染色质凝聚.应用普罗布考后细胞存活率为对照组的(92.68±5.03)%,β-半乳糖苷酶染色阳性率降至45.2%,细胞周期各期比例接近正常,细胞形态及超微结构改变减轻.结论 叔丁基过氧化氢可诱导体外培养的鼠肾小球系膜细胞发生衰老,应用普罗布考可以延缓衰老.
目的 探討叔丁基過氧化氫體外誘導鼠腎小毬繫膜細胞衰老及普囉佈攷延緩衰老的作用.方法 應用不同濃度叔丁基過氧化氫刺激鼠繫膜細胞,每天刺激1 h,連續作用4 d.刺激結束後48 h檢測細胞存活率、β-半乳糖苷酶染色暘性細胞率、細胞週期情況鑒定衰老細胞,併透射電鏡觀察衰老細胞超微結構,併觀察普囉佈攷對上述指標的影響.結果 30μmol/L叔丁基過氧化氫誘導組細胞存活率為對照組的(80.12±3.25)%(P<0.05),約81%的細胞呈現β-半乳糖苷酶染色暘性,流式細胞儀檢測86%細胞阻滯于G0、G1期,透射電鏡可見叔丁基過氧化氫誘導組細胞覈膜內陷,染色質凝聚.應用普囉佈攷後細胞存活率為對照組的(92.68±5.03)%,β-半乳糖苷酶染色暘性率降至45.2%,細胞週期各期比例接近正常,細胞形態及超微結構改變減輕.結論 叔丁基過氧化氫可誘導體外培養的鼠腎小毬繫膜細胞髮生衰老,應用普囉佈攷可以延緩衰老.
목적 탐토숙정기과양화경체외유도서신소구계막세포쇠로급보라포고연완쇠로적작용.방법 응용불동농도숙정기과양화경자격서계막세포,매천자격1 h,련속작용4 d.자격결속후48 h검측세포존활솔、β-반유당감매염색양성세포솔、세포주기정황감정쇠로세포,병투사전경관찰쇠로세포초미결구,병관찰보라포고대상술지표적영향.결과 30μmol/L숙정기과양화경유도조세포존활솔위대조조적(80.12±3.25)%(P<0.05),약81%적세포정현β-반유당감매염색양성,류식세포의검측86%세포조체우G0、G1기,투사전경가견숙정기과양화경유도조세포핵막내함,염색질응취.응용보라포고후세포존활솔위대조조적(92.68±5.03)%,β-반유당감매염색양성솔강지45.2%,세포주기각기비례접근정상,세포형태급초미결구개변감경.결론 숙정기과양화경가유도체외배양적서신소구계막세포발생쇠로,응용보라포고가이연완쇠로.
Objective To investigate the senescence of rat mesangial cells induced by Tert-Butyl hydroperoxide (tBHP) and the protective effect of probucol on senesecence. Methods Human glomerular mesangial cells(hGMC) were cultured in vitro and intervened by tBHP. The cell survival rate was observed by methyl thiazolyl tetrazolium( MTT). β-gal staining and cell cycle analysis were used to identify cell senescent status;transmission eletric microscopy was used to evaluate the ultra-microstructure of hGMC. Senescent-related indexes were detected after treatment with probucol. Results The cell survival rate with 30 μmol/L tBHP was (80. 12 ± 3. 25 ) % , the positive rate of β-gal staining was significantly higher in tBHP-induced cells (about 81% )than that of the control cells( P <0. 01). 86% of the cells was arrested at G0-G1 phase. Invagination of nucleus membrane and chromatin condensation at the nuclear margin in tBHP-induced cells was observed through transmission eletric microscopy. In the probucol intervented cells, the cell survival rate was higher than that of tBHP-induced cells (92. 68 ± 5.03) % vs. ( 80. 12 ± 3. 25) % (P < 0. 05 ). The positive rate of β-gal staining decreased to 45. 2%. The proportion of cell cycle stage was similar to the control cells.The change of morphous and ultrastructure was relieved. Conclusions tBHP can induce hGMC senescence in vitro and probucol may play a role in preventing hGMC senescence.
