中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2012年
2期
3-7
,共5页
程刚%许俊龙%张学广%李鑫%任玉波%张连群%赵存友%李学元
程剛%許俊龍%張學廣%李鑫%任玉波%張連群%趙存友%李學元
정강%허준룡%장학엄%리흠%임옥파%장련군%조존우%리학원
神经胶质瘤%增殖细胞核抗原%细胞凋亡%哺乳动物雷帕霉素靶蛋白%存活蛋白
神經膠質瘤%增殖細胞覈抗原%細胞凋亡%哺乳動物雷帕黴素靶蛋白%存活蛋白
신경효질류%증식세포핵항원%세포조망%포유동물뢰파매소파단백%존활단백
Glioma%Proliferating cell nuclear antigen%Apoptosis%Mammalian target proteinof rapamycin%Survivin
目的 研究磷酸化哺乳动物雷帕霉素靶蛋白( pmTOR)在人神经胶质瘤中的表达及其与肿瘤抗凋亡和增殖能力的关系.方法 收集人神经胶质瘤石蜡标本87例,其中Ⅰ～Ⅱ级27例、Ⅲ级24例、Ⅳ级36例,应用免疫组织化学EliVision法检测组织中pmTOR、存活蛋白(Survivin)和增殖细胞核抗原(Ki-67)的表达,分析pmTOR与神经胶质瘤病理分级的关系及其与Survivin和Ki-67的相关性.结果 pmTOR在不同级别的神经胶质瘤中均有表达,Ⅰ一Ⅱ级阳性表达率为77.8％(21/27),Ⅲ级为75.0％(18/24),Ⅳ级为72.2％ (26/36),各级别神经胶质瘤中的阳性表达率比较差异无统计学意义(P＞0.05),但其表达强度随着肿瘤病理分级的增高而升高.pmTOR与Survivin和Ki-67的表达强度呈正相关(r=0.858,P＜ 0.01；r=0.708,P＜0.01).结论 pmTOR的表达强度与神经胶质瘤的恶性程度以及细胞抗凋亡和增殖能力有关,可作为基因治疗的有效靶点.
目的 研究燐痠化哺乳動物雷帕黴素靶蛋白( pmTOR)在人神經膠質瘤中的錶達及其與腫瘤抗凋亡和增殖能力的關繫.方法 收集人神經膠質瘤石蠟標本87例,其中Ⅰ～Ⅱ級27例、Ⅲ級24例、Ⅳ級36例,應用免疫組織化學EliVision法檢測組織中pmTOR、存活蛋白(Survivin)和增殖細胞覈抗原(Ki-67)的錶達,分析pmTOR與神經膠質瘤病理分級的關繫及其與Survivin和Ki-67的相關性.結果 pmTOR在不同級彆的神經膠質瘤中均有錶達,Ⅰ一Ⅱ級暘性錶達率為77.8％(21/27),Ⅲ級為75.0％(18/24),Ⅳ級為72.2％ (26/36),各級彆神經膠質瘤中的暘性錶達率比較差異無統計學意義(P＞0.05),但其錶達彊度隨著腫瘤病理分級的增高而升高.pmTOR與Survivin和Ki-67的錶達彊度呈正相關(r=0.858,P＜ 0.01；r=0.708,P＜0.01).結論 pmTOR的錶達彊度與神經膠質瘤的噁性程度以及細胞抗凋亡和增殖能力有關,可作為基因治療的有效靶點.
목적 연구린산화포유동물뢰파매소파단백( pmTOR)재인신경효질류중적표체급기여종류항조망화증식능력적관계.방법 수집인신경효질류석사표본87례,기중Ⅰ～Ⅱ급27례、Ⅲ급24례、Ⅳ급36례,응용면역조직화학EliVision법검측조직중pmTOR、존활단백(Survivin)화증식세포핵항원(Ki-67)적표체,분석pmTOR여신경효질류병리분급적관계급기여Survivin화Ki-67적상관성.결과 pmTOR재불동급별적신경효질류중균유표체,Ⅰ일Ⅱ급양성표체솔위77.8％(21/27),Ⅲ급위75.0％(18/24),Ⅳ급위72.2％ (26/36),각급별신경효질류중적양성표체솔비교차이무통계학의의(P＞0.05),단기표체강도수착종류병리분급적증고이승고.pmTOR여Survivin화Ki-67적표체강도정정상관(r=0.858,P＜ 0.01；r=0.708,P＜0.01).결론 pmTOR적표체강도여신경효질류적악성정도이급세포항조망화증식능력유관,가작위기인치료적유효파점.
Objective To investigate the association of phosphorylation of mammalian target protein of rapamycin (pmTOR) expression with glioma malignancy grades,and the correlation of pmTOR expression with Survivin and Ki-67,which represent tumor cell anti-apoptosis ability and reproductive activity.Methods Immunohistochemistry EliVision method was employed to detect the expression of pmTOR,Survivin and Ki-67 in paraffin tissues from 87 patients with glioma (grade Ⅰ - Ⅱ 27 cases,grade Ⅲ24 cases and grade Ⅳ 36 cases).The association between positive expression rate,level of pmTOR and malignancy grades,and the correlation of its expression level with Survivin and Ki-67 were further evaluated.Results There was no significant difference in the positive expression rate of pmTOR among grade Ⅰ - Ⅱ (77.8％,21/27),grade Ⅲ (75.0％,18/24) and grade Ⅳ (72.2％,23/36) (P ＞ 0.05).However,the significant association between pmTOR expression level and malignancy grades was observed.The expression from 87 patients with glioma was significantly positively correlated with Survivin and Ki-67 expression level (r =0.858,P ＜ 0.01 ;r =0.708,P ＜ 0.01 ).Conclusions The expression level of pmTOR is associated with malignancy grades,tumor cell anti-apoptosis ability and reproductive activity.pmTOR may be served as a useful marker for predicting the biological behavior of glioma and a useful target for gene therapy.
