CAJ | 학술논문

目的：研究低氧预处理(HPC)对心肌的保护作用。方法：借助 31　P-NMR图谱技术，在模拟Langendorff离体灌流大鼠心脏的正常生理条件下，跟踪心肌高能磷酸化合物含量的动态变化。结果：在30 min低氧期，PCr、ATP 相对含量及PCr／Pi值逐渐减小，但HPC组减小的速度比对照组慢；而在复氧期， HPC组能提高心肌高能磷酸化合物含量的恢复程度，特别在复氧初期， HPC组PCr、 ATP相对含量及P Cr／Pi值立即有了恢复；在本实验中，HPC对pHi的改善不显著。结论： HPC能降低后续长时间低氧及复氧阶段的心肌能量代谢，对心肌的低氧／复氧损伤具有保护作用。
목적：연구저양예처리(HPC)대심기적보호작용。방법：차조 31　P-NMR도보기술，재모의Langendorff리체관류대서심장적정상생리조건하 ，근종심기고능린산화합물함량적동태변화。결과：재30 min저양기，PCr、ATP 상대함량급PCr／Pi치축점감소，단HPC조감소적속도비대조조만；이재복양기， HPC조능 제고심기고능린산화합물함량적회복정도，특별재복양초기， HPC조PCr、 ATP상대함량급P Cr／Pi치립즉유료회복；재본실험중，HPC대pHi적개선불현저。결론： HPC능강 저후속장시간저양급복양계단적심기능량대사，대심기적저양／복양손상구유보호작용。
To evaluate the protective effects of hypoxic precon ditioning (HPC) on myocardial energy metabolism,Langendorff-perfused hearts,ex posed to HPC,were subjected to hypoxia/reoxygrnation(5/10 min).31　P NMR was used to sequentially follow the time courses of high energy phospha tes(HEP) contents and intracellular pH(pHi) of the rat myocardium.Results: The Contents of Phosphocreatine (PCr),Adenosine Triphosphate (ATP) and the PCr/Pi (inorganic phosphate) ratios decreased during 30 min hypoxia, but the re duction was slower for the HPC group compared with Control.Reoxygenation induced recovery of myocardial HEP in both groups, HPC enhanced the recovery especially in initial stage of reoxygenation.However ,pHi change was not significant for HPC group in this experiment.Conclusio n:HPC improves the myocardial energy metabolism level during prolonged hyp oxia and subsequent reoxygenation and protects myocardium against hypoxia/reoxyg enation injury.