中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2004年
8期
1564-1565
,共2页
坐骨神经%神经再生%动作电位
坐骨神經%神經再生%動作電位
좌골신경%신경재생%동작전위
目的:比较原代培养雪旺细胞(Schwann cells,SCs)和冷冻保存的 SCs移植对损伤后坐骨神经再生的作用.方法:原代培养和液氮保存的 SCs分别移植到桥接缺损坐骨神经的硅胶管内.在移植后不同时间,硅胶管远端神经干内注射 HRP,逆行追踪背根神经节和脊髓前角的标记神经元数量;测量再生神经纤维的复合动作电位传导速度;电镜观察再生神经纤维的髓鞘形成.结果:原代培养和冷冻保存 SCs在移植后不同时间其背根神经节(术后 6周: 3967.41± 305.91与 3890.55± 315.63, t=0.55, P > 0.05;术后 8周: 4029.33± 313.80与 4184.90± 305.75, t=1.11, P > 0.05)和脊髓前角神经元 HRP标记细胞数量(术后 6周: 404.87± 40.05与 429.77± 43.40, t=1.33,P > 0.05; 术后 8周 : 474.49± 42.74与 470.99± 38.82,t=0.19,P > 0.05)、再生神经纤维的复合动作电位传导速度 (m/s)基本一致(术后 6周: 32.28± 1.96与 32.05± 2.31,t=0.24,P > 0.05; 术后 8周: 43.60± 1.88与 43.84± 2.19,t=0.26,P > 0.05; 术后 12周 : 43.78± 1.71与 43.83± 1.75,t=0.06,P > 0.05),再生神经纤维髓鞘的形成未见明显差别.结论:冷冻保存的 SCs仍具有促进损伤后周围神经再生的能力.
目的:比較原代培養雪旺細胞(Schwann cells,SCs)和冷凍保存的 SCs移植對損傷後坐骨神經再生的作用.方法:原代培養和液氮保存的 SCs分彆移植到橋接缺損坐骨神經的硅膠管內.在移植後不同時間,硅膠管遠耑神經榦內註射 HRP,逆行追蹤揹根神經節和脊髓前角的標記神經元數量;測量再生神經纖維的複閤動作電位傳導速度;電鏡觀察再生神經纖維的髓鞘形成.結果:原代培養和冷凍保存 SCs在移植後不同時間其揹根神經節(術後 6週: 3967.41± 305.91與 3890.55± 315.63, t=0.55, P > 0.05;術後 8週: 4029.33± 313.80與 4184.90± 305.75, t=1.11, P > 0.05)和脊髓前角神經元 HRP標記細胞數量(術後 6週: 404.87± 40.05與 429.77± 43.40, t=1.33,P > 0.05; 術後 8週 : 474.49± 42.74與 470.99± 38.82,t=0.19,P > 0.05)、再生神經纖維的複閤動作電位傳導速度 (m/s)基本一緻(術後 6週: 32.28± 1.96與 32.05± 2.31,t=0.24,P > 0.05; 術後 8週: 43.60± 1.88與 43.84± 2.19,t=0.26,P > 0.05; 術後 12週 : 43.78± 1.71與 43.83± 1.75,t=0.06,P > 0.05),再生神經纖維髓鞘的形成未見明顯差彆.結論:冷凍保存的 SCs仍具有促進損傷後週圍神經再生的能力.
목적:비교원대배양설왕세포(Schwann cells,SCs)화냉동보존적 SCs이식대손상후좌골신경재생적작용.방법:원대배양화액담보존적 SCs분별이식도교접결손좌골신경적규효관내.재이식후불동시간,규효관원단신경간내주사 HRP,역행추종배근신경절화척수전각적표기신경원수량;측량재생신경섬유적복합동작전위전도속도;전경관찰재생신경섬유적수초형성.결과:원대배양화냉동보존 SCs재이식후불동시간기배근신경절(술후 6주: 3967.41± 305.91여 3890.55± 315.63, t=0.55, P > 0.05;술후 8주: 4029.33± 313.80여 4184.90± 305.75, t=1.11, P > 0.05)화척수전각신경원 HRP표기세포수량(술후 6주: 404.87± 40.05여 429.77± 43.40, t=1.33,P > 0.05; 술후 8주 : 474.49± 42.74여 470.99± 38.82,t=0.19,P > 0.05)、재생신경섬유적복합동작전위전도속도 (m/s)기본일치(술후 6주: 32.28± 1.96여 32.05± 2.31,t=0.24,P > 0.05; 술후 8주: 43.60± 1.88여 43.84± 2.19,t=0.26,P > 0.05; 술후 12주 : 43.78± 1.71여 43.83± 1.75,t=0.06,P > 0.05),재생신경섬유수초적형성미견명현차별.결론:냉동보존적 SCs잉구유촉진손상후주위신경재생적능력.
AIM:To compare the effect of primary cultured Schwann cells(SCs) and cryopreserved SCs transplantation on injured sciatic nerve regeneration. METHODS:Primary cultured and liquid nitrogen kept SCs were transplanted into silica tube that bridged a sciatic nerve deficit. At different time points after the transplantation (end of 6th and 8th week after the operation), HRP was injected into the distal nerve stump in the tube. Then, the numbers of labeled neurons in dorsal root ganglion and anterior horn were traced by reversed follow-up. MCV of newly regenerated nerve fiber was measured, and myelinization of nerve fiber was studied under electron microscope. RESULTS:The number of dorsal root ganglion with primary culture and freeze kept SCs grafts at different time periods was (6 weeks after operation:3967.41± 305.91 and 3890.55± 315.63, t=0.55, P > 0.05; 8 weeks after operation: 4029.33± 313.80 and 4184.90± 305.75, t=1.11,P > 0.05) and HRP labeled neurons(6 weeks after operation: 404.87± 40.05 and 429.77± 43.40, t=1.33,P > 0.05; 8 weeks after operation: 474.49± 42.74 and 470.99± 38.82,t=0.19,P > 0.05); Both groups showed a similar MCV(6 weeks after operation: 32.28± 1.96 and 32.05± 2.31,t=0.24,P > 0.05; 8 weeks after operation: 43.60± 1.88 and 43.84± 2.19,t=0.26,P > 0.05; 12 weeks after operation: 43.78± 1.71 and 43.83± 1.75,t=0.06,P > 0.05). No apparent difference in nerve fiber myelinization was observed. CONCLUSION:Cryopreserved SCs still keep the same ability of promoting injured peripheral nerve regeneration.
