CAJ | 학술논문

目的探讨白细胞介素1β(IL-1 β)与地塞米松联合应用对人成骨细胞(Human Osteoblast-likecells HOS)芳香化酶活性的影响.方法对人的成骨细胞进行培养,设立实验组,分别加入IL-1β 1ng/mL(实验Ⅰ组)、10 ng/mL(实验Ⅱ组)、50 ng/mL(实验Ⅲ组);分别加地塞米松浓度0.1 uM(实验Ⅳ组)、1.0 uM(实验Ⅴ组)、10.0 uM(实验Ⅵ组);地塞米松浓度0.1 uM和IL-1β 1ng/mL(实验Ⅶ组);IL-1 β10 ng/mL和IL-1ra 500 ng/mL(实验Ⅷ组),对照组加培养液,采用氚水法检测芳香化酶活性.结果实验组Ⅰ,Ⅱ,Ⅲ组氚水放射强度与对照组比较放射强度明显增加,差异显著(P＜0 05).实验Ⅳ,Ⅴ,Ⅵ组放射强度与对照组比较有非常显著性差异(P＜0.001).实验Ⅶ组与对照组间差异无统计学意义.实验Ⅷ组放射强度高于对照组及实验组Ⅰ和Ⅳ,差异非常显著(P＜0.001).结论人成骨细胞中IL-1 β、地塞米松分别可以增加芳香化酶的活性.IL-1β是通过与成骨细胞的IL-1受体相结合而起作用.地塞米松和IL-1β联合应用使芳香化酶活性增加,它们相互作用影响着骨骼中雌激素合成.
목적탐토백세포개소1β(IL-1 β)여지새미송연합응용대인성골세포(Human Osteoblast-likecells HOS)방향화매활성적영향.방법대인적성골세포진행배양,설립실험조,분별가입IL-1β 1ng/mL(실험Ⅰ조)、10 ng/mL(실험Ⅱ조)、50 ng/mL(실험Ⅲ조);분별가지새미송농도0.1 uM(실험Ⅳ조)、1.0 uM(실험Ⅴ조)、10.0 uM(실험Ⅵ조);지새미송농도0.1 uM화IL-1β 1ng/mL(실험Ⅶ조);IL-1 β10 ng/mL화IL-1ra 500 ng/mL(실험Ⅷ조),대조조가배양액,채용천수법검측방향화매활성.결과실험조Ⅰ,Ⅱ,Ⅲ조천수방사강도여대조조비교방사강도명현증가,차이현저(P＜0 05).실험Ⅳ,Ⅴ,Ⅵ조방사강도여대조조비교유비상현저성차이(P＜0.001).실험Ⅶ조여대조조간차이무통계학의의.실험Ⅷ조방사강도고우대조조급실험조Ⅰ화Ⅳ,차이비상현저(P＜0.001).결론인성골세포중IL-1 β、지새미송분별가이증가방향화매적활성.IL-1β시통과여성골세포적IL-1수체상결합이기작용.지새미송화IL-1β연합응용사방향화매활성증가,타문상호작용영향착골격중자격소합성.
[Objective] To study the stimulation of Interleukin-1β (IL-1β) and Dexamethasone on aromatase activity of human osteoblast-like cells (HOS). [Methods] In the present study, the effect of IL-1β and Dexamethasone on aromatase activity was investigated by measuring [3H] water released upon the conversion of [1β- 3H] androstenedione to estrone. The experimental group was divided into nine group: G1 (IL-1β 1 ng/ml), G2 (IL-1β 10ng/ml), G3 (IL-1β 50 ng/ml), G4 (Dexamethesone 0.1 uM), G5 (Dexamethesone 1 uM), G6 (Dexamethesone 10 uM),G7 (Dexamethesone 0.1 uM and IL-1β 1 ng/ml), G8 (IL-1β 10 ng/ml and IL-1ra 500 ng/ml) and one control group.[Results] Administration of IL-1β or Dexamethasone increased aromatase activity by dose dependent manner.However, addition of IL-1β 1 ng/ml with Dexametheson 0.1uM resulted in 110% greater than control. Addition of 500 ng/ml of human recombinant IL-1 receptor antagonist (IL-1ra) neutralized the increased aromatase activity to control level. [ Conclusions] These findings suggest that IL-1β and Dexamethasone stimulated the aromatase activity in osteoblast-like cells by promoting the estrogen synthesis and IL-1β regulates it through the IL-1 receptor.Meanwhile, the presence of Dexamethasone is necessary for the induction of aromatase. Both of them may be involved in the regulatory mechanism for estrogen formation in bone.