CAJ | 학술논문

从制丝厂的废水池中分离获得39个可以降解家蚕丝胶蛋白的细菌分离株,采用考马斯亮蓝G-250法测定各菌株对丝胶蛋白的降解活性,降解率介于2.69%～28.86%之间,各菌株间的降解活性存在显著差异(P＜0.000 1),以X10菌株的降解活性最高.采用rep-PCR技术对39个细菌分离株进行聚类分析,在rep-PCR图谱的相异百分数为0.9的水平上被聚类为12个类群,39个分离株各自具有独特的带型,说明各细菌分离株的基因组之间存在显著异质性,表现出丰富的基因型多态性.分离这些丝胶蛋白降解细菌是为了进一步筛选后用于制丝厂的废水处理.
종제사엄적폐수지중분리획득39개가이강해가잠사효단백적세균분리주,채용고마사량람G-250법측정각균주대사효단백적강해활성,강해솔개우2.69%～28.86%지간,각균주간적강해활성존재현저차이(P＜0.000 1),이X10균주적강해활성최고.채용rep-PCR기술대39개세균분리주진행취류분석,재rep-PCR도보적상이백분수위0.9적수평상피취류위12개류군,39개분리주각자구유독특적대형,설명각세균분리주적기인조지간존재현저이질성,표현출봉부적기인형다태성.분리저사사효단백강해세균시위료진일보사선후용우제사엄적폐수처리.
39 bacterial isolates that had sericin degradation activity were obtained from the reeling mill waste water. Their sericin degradation activities were determined by Coomassie Brilliant Blue G-250 method. The degradation rates of these isolates were between 2.69% and 28.86%. The degradation activities were significantly different (P＜0.000 1) between each isolate. The highest degradation activity was found in the X10 isolate. rep-PCR method was used to conduct cluster analysis to the 39 bacterial isolates. rep-PCR analysis revealed that the 39 bacterial isolates could be divided into 12 clusters at the 0.9 dissimilarity percentage level. The 39 bacterial isolates had their own specific band patterns, showing obvious genomic heterogenicity and rich genotypic polymorphism genetic diversity between each isolate. After further screening, these sericin degradation bacteria can be used to treat the waste water of a reeling mill.