CAJ | 학술논문

运用生物信息学技术比较Ara h2和Am h6从一级结构到三级结构的异同.结果发现,Am h2含有一段独有的序列(60～73),其中包括Ara h2三个主要IgE抗原表位中的两个.以Ara h6为模板进行同源建模获得了Ara h2的立体结构,与Ara h6的结构叠加拟合后,该结构多出一段由蛋白环连接的反向平行β-折叠(58～72),其伸展结构同样包含上述两个IgE表位的蛋白序列.探讨从Ara h2和Ara h6的一级结构到三级结构产生免疫学活性差异的原因,为研究花生过敏机制及设计低致敏原疫苗奠定基础.
운용생물신식학기술비교Ara h2화Am h6종일급결구도삼급결구적이동.결과발현,Am h2함유일단독유적서렬(60～73),기중포괄Ara h2삼개주요IgE항원표위중적량개.이Ara h6위모판진행동원건모획득료Ara h2적입체결구,여Ara h6적결구첩가의합후,해결구다출일단유단백배련접적반향평행β-절첩(58～72),기신전결구동양포함상술량개IgE표위적단백서렬.탐토종Ara h2화Ara h6적일급결구도삼급결구산생면역학활성차이적원인,위연구화생과민궤제급설계저치민원역묘전정기출.
The allergens Ara h2 and Ara h6 are the most clinically relevant allergens of peanut allergies.Ara h2 can completely inhibit the IgE binding ability of Ara h6 while Ara h6 can only partially inhibit IgE epitope of Ara h2.Comparison between the primary and tertiary structures of Ara h2 and Ara h6 is carried out for the exploration of this mechanism.Ara h2 contains a unique fragment (from 60 to 73) which includes two of the three major linear IgE epitopes of Ara h2.A 3-D structure of Ara h2 is obtained by homology modeling with Ara h6 as the template.When the structure of Ara h2 and Ara h6 are superposed,an extra outstretched anti-parallel β-sheet linked a loop (from 58 to 72) is found within the structure of Ara h2.It also contains the sequence encoding the above-mentioned two IgE epitopes.This study gives a explanation for the difference of Ara h2 and Ara h6 by comparison of primary and tertiary structures of Ara h2 and Ara h6. The explanation lays down the foundation for understanding of the mechanisms of peanut allergies and future development of hypoallergic vaccines.