CAJ | 학술논문

目的观察诊断超声联合不同剂量超声造影剂对正常关节滑膜组织的生物学作用. 方法 20只正常清洁级Wister大鼠,分5组(每组4只,8个膝关节):超声+100 μl造影剂、超声+200 μl造影剂、超声+300 μl造影剂、超声+500 μl造影剂、单纯超声照射(对照组).各组均使用诊断超声持续照射10 min.1周后处死大鼠,取关节滑膜组织行HE染色,观察大鼠关节滑膜组织有无损伤及损伤情况. 结果超声+100 μl造影剂、超声+200 μl造影剂及超声+300 μl造影剂、单纯超声照射各组未发现关节滑膜组织有明显损伤.在超声+500 μl造影剂组发现滑膜细胞肿胀,呈圆形、椭圆形或多边形,滑膜组织增生明显,层次明显增多,排列紊乱,周围组织中毛细血管增生明显,部分肌纤维结构溶解断裂,炎性细胞浸润. 结论超声联合造影剂能明显增强对滑膜组织的生物学效应,且造影剂剂量越大,滑膜组织损伤越严重.
목적 관찰진단초성연합불동제량초성조영제대정상관절활막조직적생물학작용. 방법 20지정상청길급Wister대서,분5조(매조4지,8개슬관절):초성+100 μl조영제、초성+200 μl조영제、초성+300 μl조영제、초성+500 μl조영제、단순초성조사(대조조).각조균사용진단초성지속조사10 min.1주후처사대서,취관절활막조직행HE염색,관찰대서관절활막조직유무손상급손상정황. 결과 초성+100 μl조영제、초성+200 μl조영제급초성+300 μl조영제、단순초성조사각조미발현관절활막조직유명현손상.재초성+500 μl조영제조발현활막세포종창,정원형、타원형혹다변형,활막조직증생명현,층차명현증다,배렬문란,주위조직중모세혈관증생명현,부분기섬유결구용해단렬,염성세포침윤. 결론 초성연합조영제능명현증강대활막조직적생물학효응,차조영제제량월대,활막조직손상월엄중.
Objective To observe the bioeffects on the normal rats synovial with diagnostic ultrasound combined with different doses contrast medium. Methods Twenty normal cleaning level Wister rats were divided into 5 groups (each with 4 rats and 8 knees): ultrasound+100 μl contrast medium, ultrasound+200 μl contrast medium, ultrasound+300 μl contrast medium, ultrasound+500 μl contrast medium, a simple ultrasound irradiation (control group). Each group was irradiated with diagnostic ultrasound for 10 min. Rats were sacrificed after 1 week and joints synovial tissues were observed with pathological HE staining. Results In group ultrasound+100 μl contrast medium, ultrasound+200 μl contrast medium, ultrasound+300 μl contrast medium and control group, no significant damage in synovial tissues was found. In group ultrasound+500 μl contrast medium, synovial cells swelling, showing round, oval or polygonal, synovial tissues hyperplasia, arranged disorder, capillary proliferation, some muscle fiber structure dissolved fracture, inflammatory cell infiltration in the surrounding tissue were found. Conclusion Ultrasound combined with contrast medium can significantly enhance the bioeffects of synovial tissues, and the more the contrast medium doses, the more serious injury occurs in synovial tissues.