CAJ | 학술논문

目的初步确定广州地区健康学龄前儿童外周血CD4+CD25nt/hiCD127lo/Treg细胞的表达,建立学龄前儿童CD4+CD25nt/hiCD127loTreg细胞的正常参考范围,为临床研究相关疾病提供参考依据.方法收集150名3～6岁体检健康的学龄前儿童外周血,采用多色免疫荧光素标记和流式细胞术检测外周血中CD4+CD25nt/hiCD127loTreg细胞的表达.结果健康学龄前儿童CD4+CD25nt/hiCD127loTreg细胞在外周血中约占CD4+T细胞的6.52％～6.97％,不同性别表达差异无显著性.结论初步确定广州地区学龄前健康儿童CD4+CD25nt/hiCD127loTreg细胞在外周血中的正常范围,为临床研究儿童相关疾病提供参考依据.应用IL-7受体细胞膜表面标志物CD127联合检测外周血CD4+CD25nt/hiCD127loTreg细胞,能较好地反映完整的调节性Treg细胞.
목적 초보학정엄주지구건강학령전인동외주혈CD4+CD25nt/hiCD127lo/Treg세포적표체,건립학령전인동CD4+CD25nt/hiCD127loTreg세포적정상삼고범위,위림상연구상관질병제공삼고의거.방법 수집150명3～6세체검건강적학령전인동외주혈,채용다색면역형광소표기화류식세포술검측외주혈중CD4+CD25nt/hiCD127loTreg세포적표체.결과 건강학령전인동CD4+CD25nt/hiCD127loTreg세포재외주혈중약점CD4+T세포적6.52％～6.97％,불동성별표체차이무현저성.결론 초보학정엄주지구학령전건강인동CD4+CD25nt/hiCD127loTreg세포재외주혈중적정상범위,위림상연구인동상관질병제공삼고의거.응용IL-7수체세포막표면표지물CD127연합검측외주혈CD4+CD25nt/hiCD127loTreg세포,능교호지반영완정적조절성Treg세포.
Objective To determine the CD4+CD25nt/hiCD127lo regulatory T cell(Treg) from the peripheral blood of healthy preschool children in Guangzhou and provide the reference for the clinical research on correlative diseases.Methods Using multicolor immunofluorescence flow-cytometry to detect the CD4+CD25nt/b1CD127lo Treg 150 healthy preschool children aged from 3～6 years with different gender.Results The percentage of the CD4+CD25nt/hiCD 127lo Treg in preschool children was (6.52～6.97)％,there was no significant difference between 2 genders.Conclusion CD4+CD25nt/h1CD127lo Treg from the peripheral blood of healthy preschool children has been determined which provides the reference for the clinical research about correlative diseases,using the IL-7 receptor (CD127),the surface marker of IL-7receptor combined with CD4+CD25nt/hiCD 127lo Treg cell can reflect the proportion of Treg.