CAJ | 학술논문

目的观察两种中药提取物黄芩苷、京尼平苷对多药耐药(MDR)K562/ADM细胞的耐药逆转作用,探讨其分子机制.方法建立阿霉素(ADM)诱导K562/ADM细胞耐药模型,分别将不同浓度黄芩苷、京尼平苷作用于K562/ADM细胞,用四甲基偶氮唑盐(MTT)法检测其对化疗药物的敏感性,用逆转录聚合酶链反应(RT-PCR)检测多药耐药基因-1(mdr-1)、拓扑异构酶Ⅱ(TopoⅡ)基因在mRNA水平的表达变化.结果两种中药提取物黄芩苷、京尼平苷,可增加K562/ADM细胞对ADM的敏感性,K562/ADM细胞增殖受到明显抑制,耐药逆转倍数分别为1.95倍及1.46倍;RT-PCR检测mdr-1基因表达减弱,Topo Ⅱ基因表达增强(P<0.01).结论黄芩苷、京尼平苷可通过降低K562/ADM细胞mdr-1基因表达和提高TopoⅡ的表达来逆转白血病细胞MDR.
목적 관찰량충중약제취물황금감、경니평감대다약내약(MDR)K562/ADM세포적내약역전작용,탐토기분자궤제.방법 건립아매소(ADM)유도K562/ADM세포내약모형,분별장불동농도황금감、경니평감작용우K562/ADM세포,용사갑기우담서염(MTT)법검측기대화료약물적민감성,용역전록취합매련반응(RT-PCR)검측다약내약기인-1(mdr-1)、탁복이구매Ⅱ(TopoⅡ)기인재mRNA수평적표체변화.결과 량충중약제취물황금감、경니평감,가증가K562/ADM세포대ADM적민감성,K562/ADM세포증식수도명현억제,내약역전배수분별위1.95배급1.46배;RT-PCR검측mdr-1기인표체감약,Topo Ⅱ기인표체증강(P<0.01).결론 황금감、경니평감가통과강저K562/ADM세포mdr-1기인표체화제고TopoⅡ적표체래역전백혈병세포MDR.
Objective To investigate the reversal effect of the monomer of traditional Chinese medicine on muhidrug resistance(MDR) and its possible mechanism in K562/ADM cell line in vitro. Methods With different concentrations of baicalin, geniposide administered to K562/ADM cells, the proliferation of K562/ ADM cells was detected by the MTY assay. Expression of mdr-1 mRNA, Topo Ⅱ mRNA was measured by semi-quantitive RT-PCR. Results Thatbaicalin and geniposide could increase the sensitivity of K562/ADM cells to adriamycin, multiples of reversion were 1.95 times and 1.46 times. The proliferation of K562/ADM cells was in-hibited obviously by baicalin and geniposide, the level of mdr-1 mRNA expression was down-regulated and the Topo Ⅱ mRNA was up-regulated(P<0.01 ). Conclusion Baicalin and geniposide may reverse the multi-drag-resistance of K562/ADM cells, which was related to the down-regulation of mdr-1 expression and up-reg-ulation of Topo Ⅱ beta expression.