CAJ | 학술논문

目的探讨肺功能检查专用呼吸过滤器细菌过滤效率的检测方法,并评价其可行性.方法采用射流雾化器产生带有枯草芽孢杆菌黑色变种标准菌种细菌悬液(浓度为1.5×108 cfu/ml)的气溶胶,分别通过3种不同品牌的呼吸过滤器,然后采集过滤器末端的气流进行细菌培养和菌落计数,计算出呼吸过滤器的细菌过滤效率和微生物穿透值.结果 Promed、Microgard和Cosmed 3种过滤器的细菌过滤效率均大于99.99%,各组间差异无统计学意义(P＞0.05).微生物穿透值分别为(17±9)、(23±12)和(13±8),3组间及Promed与其余2组间差异无统计学意义(P＞0.05).结论通过雾化细菌悬液检测肺功能检查专用呼吸过滤器细菌过滤效率的方法可行.国内产品(Promed)与国内常用进口产品(Microgard和Cosmed)的过滤性能相当,达到呼吸保护装置的过滤要求,适于临床上应用.
목적 탐토폐공능검사전용호흡과려기세균과려효솔적검측방법,병평개기가행성.방법 채용사류무화기산생대유고초아포간균흑색변충표준균충세균현액(농도위1.5×108 cfu/ml)적기용효,분별통과3충불동품패적호흡과려기,연후채집과려기말단적기류진행세균배양화균락계수,계산출호흡과려기적세균과려효솔화미생물천투치.결과 Promed、Microgard화Cosmed 3충과려기적세균과려효솔균대우99.99%,각조간차이무통계학의의(P＞0.05).미생물천투치분별위(17±9)、(23±12)화(13±8),3조간급Promed여기여2조간차이무통계학의의(P＞0.05).결론 통과무화세균현액검측폐공능검사전용호흡과려기세균과려효솔적방법가행.국내산품(Promed)여국내상용진구산품(Microgard화Cosmed)적과려성능상당,체도호흡보호장치적과려요구,괄우림상상응용.
Objective To develop an efficiency measurement for bacterial filtration of respiratory filters used in pulmonary function tests to and evaluate its feasibility. Methods An aerosol was generated by jet atomizer using nebulized standard bacterial suspension containing 1.5 × 108 cfu/ml Bacillus subtilis var.niger. The aerosol was driven through three types of commercial respiratory filters. The efferent flow from the filter was collected for bacterial culture and colony count. Bacterial filtration efficiency (BFE) and microbial penetration values (MPV) were computed. Results All the three filters (manufactured by Promed, Cosmed and Microgard) performed a filtration efficiency greater than 99.99% with no significant differences (P＞0.05 ). The microbial penetration values of the three filters were ( 17 ±9), ( 23 ± 12) and ( 13 ±8) respectively.There was no significant difference among the groups, or between Promed filters and the other two types (P＞0.05). Conclusions The use of nebulized bacterial suspension appears feasible to assess the bacterial filtration efficiency of respiratory filters used in pulmonary function test. Homemade filters (Promed) exhibit comparable filtration performance as do imported filters (Microgard and Cosmed) , which meet the requirement for respiratory protective devices and justify their clinical application.