CAJ | 학술논문

建立链脲佐菌素诱导的糖尿病大鼠模型,用免疫组化法观察肾小管间质血管内皮生长因子(VEGF)和色素上皮衍生因子(PEDF)的表达变化;体外培养大鼠肾成纤维细胞(NRK),用RT-PCR检测高糖对VEGF和PEDF mRNA表达的影响.结果显示糖尿病大鼠肾脏组织VEGF表达增加,而PEDF表达下降.在NRK细胞,高糖呈时间、剂量依赖性地增加VEGF和抑制PEDF的mRNA表达.提示VEGF和PEDF的表达失衡可能在糖尿病肾病的发生发展中起一定作用.
건립련뇨좌균소유도적당뇨병대서모형,용면역조화법관찰신소관간질혈관내피생장인자(VEGF)화색소상피연생인자(PEDF)적표체변화;체외배양대서신성섬유세포(NRK),용RT-PCR검측고당대VEGF화PEDF mRNA표체적영향.결과 현시당뇨병대서신장조직VEGF표체증가,이PEDF표체하강.재NRK세포,고당정시간、제량의뢰성지증가VEGF화억제PEDF적mRNA표체.제시VEGF화PEDF적표체실형가능재당뇨병신병적발생발전중기일정작용.
The expressions of vascular endothelial growth factor(VEGF)and pigment epithelium derived factor(PEDF)in the tubulointerstitium were determined by immunohistochemistry in the streptozotoein-induced diahetic rats.The mRNA expressions of VEGF and PEDF in normal rat kidney (NRK) were measured by using RT-PCR under different high glucose conditions.The results showed that VEGF expression increased and PEDF expression decreased in the kidney tissue of the streptozotocin-induced diabetic rats.Hiish glucose increased VEGF expression and decreased PEDF expression in N RK cells in a time-and dose-dependent manner.The unbalanced expressions of VEGF and PEDF may play an important role in the occurrence and progression of diabetic nephropathy.