CAJ | 학술논문

目的观察去铁敏对大鼠脑室出血(IVH)后脑积水的干预效果及脑组织Wnt1、Wnt3a的表达影响.方法雄性SD大鼠130只随机分为正常组、假性IVH组、IVH组及IVH加去铁敏组.采用脑室出血后慢性脑积水大鼠模型,于术后1d、7d及28 d处死大鼠,观察脑积水的发生率和脑组织中Wnt基因及蛋白表达情况.结果正常组、假性IVH组未见脑积水发生,IVH组28 d脑积水发生率80％ (12/15),显著高于IVH加去铁敏组28 d脑积水发生率20％ (3/15).IVH后Wnt1、Wnt3amRNA及蛋白表达在7d及28 d均显著高于假性IVH组,而去铁敏治疗组Wnt1、WnGa mRNA及蛋白表达在28 d较IVH组均显著降低.结论去铁敏治疗可降低大鼠IVH后慢性脑积水发生率,Wnt信号通路参与了IVH后脑积水发生及去铁敏干预机制.
목적 관찰거철민대대서뇌실출혈(IVH)후뇌적수적간예효과급뇌조직Wnt1、Wnt3a적표체영향.방법 웅성SD대서130지수궤분위정상조、가성IVH조、IVH조급IVH가거철민조.채용뇌실출혈후만성뇌적수대서모형,우술후1d、7d급28 d처사대서,관찰뇌적수적발생솔화뇌조직중Wnt기인급단백표체정황.결과 정상조、가성IVH조미견뇌적수발생,IVH조28 d뇌적수발생솔80％ (12/15),현저고우IVH가거철민조28 d뇌적수발생솔20％ (3/15).IVH후Wnt1、Wnt3amRNA급단백표체재7d급28 d균현저고우가성IVH조,이거철민치료조Wnt1、WnGa mRNA급단백표체재28 d교IVH조균현저강저.결론 거철민치료가강저대서IVH후만성뇌적수발생솔,Wnt신호통로삼여료IVH후뇌적수발생급거철민간예궤제.
Objective To observe the effect of deferoxamine on chronic hydrocephalus after intraventricular hemorrhage (IVH) and the role of Wnt (Wnt1 and Wnt3a).Methods A total of 130 Sprague Dawley male rats were randomly assigned into 4 groups:normal control group,sham- IVH group,IVH group and deferoxamine -treated group.The rats of subgroups except normal control group received an injection of autologous blood or saline into right lateral cerebral ventricles.Deferoxamine or vehicle was administered 3 hours after IVH and then every 12 hours up to 7 days in IVH group and deferoxamine -treated group.Rats were euthanized at 1,7,28 days for measurement,and the transverse diameter of the lateral ventricles were observed for evaluation of hydrocephalus.The expression of Wntl and Wnt3a were measured by RT - PCR and Western Blot.Results At 28 days,there was no hydrocephalus in the normal control group and sham - IVH group.80 ％ (12/15) of the rats in IVH group developed hydrocephalus.The rate was only 20 ％ (3/15) in deferoxamine - treated group,which was much lower than that in IVH group.The expression of Wnt1 mRNA was normal in normal control group and shame -IVH group,and up-regulated in IVH group.The peak expression was detected at 28 days.The expression was significantly higher than that in shame - IVH group at 7 days and 28 days,after deferoxamine treatment,downregnlation of Wnt1 mRNA were observed and were significantly lower than that in IVH group.The Wnt3a mRNA had similar trends,while the expressed level was normal in normal control group and shame - IVH group,and up -regulated following IVH.The peak expression was detected at 28 days,and was significantly higher than that in shame - IVH group at 7 days and 28 days.After deferoxamine treatment,the downregulation of Wnt3a mRNA was observed and was significantly lower than that in IVH group.Accordingly,Wntl protein expression was normal in normal control group and shame - IVH group,and increased after IVH.At 7 days and 28 days,the Wnt1 protein expression of IVH group was markedly higher than shame - IVH group.After deferoxamine treatment,the expression of Wnt1 protein was reduced and significantly lower than that of IVH group.The expression of Wnt3a protein was also normal in normal control group and shame - IVH group.The up - regulation of the protein expression was observed following IVH at 28 days,while down - regulation was observed after deferoxamine treatment at 28 days.Conclusions Deferoxamine could reduce hydrocephalus after IVH,and Wnt pathway may play an important role in the development of IVH and therapeutic effect of deferoxamine.