CAJ | 학술논문

目的应用肝螺杆菌感染不同品系小鼠,观察肝螺杆菌的定植状况及病理特征,以期获得稳定的动物模型.方法 SPF级雄性BABL/c Cr、SCID/Cr、C57BL/6 Cr小鼠接种肝螺杆菌(H.hepaticus,Hh)标准菌株ATCC51450菌液0.2 ml(1×108 CUF/ml),连续3次,每次间隔48 h,对照组灌饲等量的PBS.于末次接种Hh后第4周、8周及16周处死小鼠,取出小鼠食管、胃、空肠、回肠、盲肠、结肠、肝脏及胰腺组织,行病理组织学检查、微需氧菌分离培养鉴定及Hh特异性16SrRNA基因扩增.结果 BALB/c Cr小鼠和SCID/ Cr小鼠接种Hh后4周、8周及16周盲肠定植率均为8/8,4周、8周及16周结肠定植率分别为(4/8、5/8、5/8)和(3/8、6/8、5/8),16周回肠及空肠定植率均为1/8,8周、16周肝脏定植率分别为(2/8、3/8)和(2/8、2/8);C57BL/6 Cr小鼠接种Hh后4周、8周16周盲肠定植率分别为1/8、2/8和2/8,8周、16周结肠定植率分别为1/8、2/8.Hh感染的BALB/c Cr和SCID/Cr小鼠肝脏、盲肠及结肠炎症改变较C57BL／6 Cr小鼠更为明显(P＜0.01),且随着感染时间的延长病理组织学评分逐渐增加(P值分别＜0.05和0.01),其中结肠及肝脏组织中Hh定植者的组织学评分明显高于无Hh定植者(P＜0.05),盲肠组织的病理组织学评分随着Hh定植密度的增加而增加(P＜0.05).结论不同品系小鼠对Hh的易感性不同；BALB/cCr、SCID/Cr小鼠接种Hh后可获得较好的Hh感染模型.
목적 응용간라간균감염불동품계소서,관찰간라간균적정식상황급병리특정,이기획득은정적동물모형.방법 SPF급웅성BABL/c Cr、SCID/Cr、C57BL/6 Cr소서접충간라간균(H.hepaticus,Hh)표준균주ATCC51450균액0.2 ml(1×108 CUF/ml),련속3차,매차간격48 h,대조조관사등량적PBS.우말차접충Hh후제4주、8주급16주처사소서,취출소서식관、위、공장、회장、맹장、결장、간장급이선조직,행병리조직학검사、미수양균분리배양감정급Hh특이성16SrRNA기인확증.결과 BALB/c Cr소서화SCID/ Cr소서접충Hh후4주、8주급16주맹장정식솔균위8/8,4주、8주급16주결장정식솔분별위(4/8、5/8、5/8)화(3/8、6/8、5/8),16주회장급공장정식솔균위1/8,8주、16주간장정식솔분별위(2/8、3/8)화(2/8、2/8);C57BL/6 Cr소서접충Hh후4주、8주16주맹장정식솔분별위1/8、2/8화2/8,8주、16주결장정식솔분별위1/8、2/8.Hh감염적BALB/c Cr화SCID/Cr소서간장、맹장급결장염증개변교C57BL／6 Cr소서경위명현(P＜0.01),차수착감염시간적연장병리조직학평분축점증가(P치분별＜0.05화0.01),기중결장급간장조직중Hh정식자적조직학평분명현고우무Hh정식자(P＜0.05),맹장조직적병리조직학평분수착Hh정식밀도적증가이증가(P＜0.05).결론 불동품계소서대Hh적역감성불동；BALB/cCr、SCID/Cr소서접충Hh후가획득교호적Hh감염모형.
Objective To obtain stable animal models and observe Helicobacter hepaticas (Hh)colonization and pathological claracteristics,through infecting different mice strains with Hh.Methods SPF-class male BABL/c Cr,SCID/Cr and C57BL/6 Cr mice were inoculated 0.2 ml Hh standard strain ATCC51450 bacterial suspension (1 × 108CUF/ml),inoculated for 3 times with 48 hours intervals,the control group was fed with the same volume of PBS.Mice were executed at 4 weeks,8weeks and 16 weeks since last Hh inoculation,and mice esophagus,stomach,jejunum,ileum,cecum,colon,liver and pancreas tissue were taken for histopathology examination,Micro-aerobic bacteria isolation,culture and identification and Hh specific 16S rRNA gene amplification.Results The colonization rates of Hh in cecum after inoculated in BALB/c Cr mice and SCID/Cr mice at 4 weeks,8 weeks and 16 weeks were all 8/8,colonization rates in colon at 4 weeks,8 weeks and 16 weeks were 4/8,5/8,5/8 and 3/8,6/8,5/8 respectively,colonization rates in ileum and jejunum at 16 weeks were 1/8,colonization rates in liver at 8 weeks and 16 weeks were 2/8,3/8 and 2/8,2/8respectively.The colonization rates of Hh in cecum after inoculated in C57BL/6 Cr mice at 4 weeks,8weeks and 16 weeks were 1/8,2/8 and 2/8 respectively,colonization rates in colon at 8 weeks and 16weeks were 1/8,2/8 respectively.Compared with C57BL/6 Cr mice,the inflammatory changes in liver,cecum and colon were more significant in Hh infected BALB/c Cr and SCID/Cr mice (P＜0.01),and histological scores gradually increased as infection time extended (P＜0.05,P＜ 0.01 ).The histological scores were significantly higher in those with colon and liver Hh bacterial colonization than those without Hh bacterial colonization (P＜0.05).The histopathological score of cecal tissue was positively correlated with the density of Hh colonization.Conclusion Different mice strains are with different susceptibility to Hh,and better Hh infection model can be obtained in Hh inoculated BALB/c Cr and SCID/Cr mice.