肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2007年
z1期
23-27
,共5页
人乳头瘤病毒%子宫疾病%原位杂交
人乳頭瘤病毒%子宮疾病%原位雜交
인유두류병독%자궁질병%원위잡교
Human papillomavirus(HPV)%Uterine diseases%In situ hybridization
目的 通过检测低危型HPV6/11及高危型HPV16/18在慢性子宫颈炎、子宫颈尖锐湿疣不伴非典型增生、子宫颈鳞状上皮CIN Ⅰ级、子宫颈鳞状上皮CIN Ⅲ级、子宫颈浸润性鳞状细胞癌五种子宫颈病变中的表达情况,探讨HPV与子宫颈病变的相关性、作用机制及其临床意义.方法 应用组织芯片技术,将150例子宫颈病变患者的标本制成组织芯片,用原位杂交法对其进行6/11型及16/18型HPV的测定,应用SPSS 10.0统计软件进行数据分析.结果 低危型HPV6/11在慢性子宫颈炎、子宫颈尖锐湿疣、子宫颈鳞状上皮CIN Ⅰ级、子宫颈鳞状上皮CIN Ⅲ级、子宫颈浸润性鳞状细胞癌中的阳性表达率分别为:13.33%、90%、33.33%、0、0,其中尖锐湿疣组HPV6/11的阳性表达率显著高于其他各组(均P＜0.001);CIN Ⅰ组与CIN Ⅲ及癌比较差异有统计学意义(P=0.001,＜0.05);运用Spearman相关分析表明,HPV6/11在上述五种病变中的阳性表达率与恶性度呈负相关(rs=-0.370,P＜0.001).高危型HPV16/18在慢性子宫颈炎、尖锐湿疣、CIN Ⅰ级、CIN Ⅲ级、鳞状细胞癌中的阳性表达率分别为0、6.67%、10%、56.67%、76.67%,CIN Ⅲ及鳞癌与其他各组间比较均有统计学意义(P＜0.001),但CIN Ⅲ与鳞癌之间无统计学意义(P=0.10);运用Spearman相关分析表明,HPV16/18在上述五种病变中的阳性表达率与恶性度呈正相关(rs=0.628,P＜0.001).结论 低危型HPV6/11主要引起子宫颈尖锐湿疣及CIN Ⅰ;高危型HPV16/18与CIN Ⅲ及子宫颈浸润性鳞癌关系密切,是引起子宫颈CIN Ⅲ及浸润性癌的主要因素.对HPV在子宫颈病变中检测及分型有助于对子宫颈病变的诊断和监测,尤其是对子宫颈癌的预防、早期诊断及早期治疗具有重要意义.
目的 通過檢測低危型HPV6/11及高危型HPV16/18在慢性子宮頸炎、子宮頸尖銳濕疣不伴非典型增生、子宮頸鱗狀上皮CIN Ⅰ級、子宮頸鱗狀上皮CIN Ⅲ級、子宮頸浸潤性鱗狀細胞癌五種子宮頸病變中的錶達情況,探討HPV與子宮頸病變的相關性、作用機製及其臨床意義.方法 應用組織芯片技術,將150例子宮頸病變患者的標本製成組織芯片,用原位雜交法對其進行6/11型及16/18型HPV的測定,應用SPSS 10.0統計軟件進行數據分析.結果 低危型HPV6/11在慢性子宮頸炎、子宮頸尖銳濕疣、子宮頸鱗狀上皮CIN Ⅰ級、子宮頸鱗狀上皮CIN Ⅲ級、子宮頸浸潤性鱗狀細胞癌中的暘性錶達率分彆為:13.33%、90%、33.33%、0、0,其中尖銳濕疣組HPV6/11的暘性錶達率顯著高于其他各組(均P＜0.001);CIN Ⅰ組與CIN Ⅲ及癌比較差異有統計學意義(P=0.001,＜0.05);運用Spearman相關分析錶明,HPV6/11在上述五種病變中的暘性錶達率與噁性度呈負相關(rs=-0.370,P＜0.001).高危型HPV16/18在慢性子宮頸炎、尖銳濕疣、CIN Ⅰ級、CIN Ⅲ級、鱗狀細胞癌中的暘性錶達率分彆為0、6.67%、10%、56.67%、76.67%,CIN Ⅲ及鱗癌與其他各組間比較均有統計學意義(P＜0.001),但CIN Ⅲ與鱗癌之間無統計學意義(P=0.10);運用Spearman相關分析錶明,HPV16/18在上述五種病變中的暘性錶達率與噁性度呈正相關(rs=0.628,P＜0.001).結論 低危型HPV6/11主要引起子宮頸尖銳濕疣及CIN Ⅰ;高危型HPV16/18與CIN Ⅲ及子宮頸浸潤性鱗癌關繫密切,是引起子宮頸CIN Ⅲ及浸潤性癌的主要因素.對HPV在子宮頸病變中檢測及分型有助于對子宮頸病變的診斷和鑑測,尤其是對子宮頸癌的預防、早期診斷及早期治療具有重要意義.
목적 통과검측저위형HPV6/11급고위형HPV16/18재만성자궁경염、자궁경첨예습우불반비전형증생、자궁경린상상피CIN Ⅰ급、자궁경린상상피CIN Ⅲ급、자궁경침윤성린상세포암오충자궁경병변중적표체정황,탐토HPV여자궁경병변적상관성、작용궤제급기림상의의.방법 응용조직심편기술,장150례자궁경병변환자적표본제성조직심편,용원위잡교법대기진행6/11형급16/18형HPV적측정,응용SPSS 10.0통계연건진행수거분석.결과 저위형HPV6/11재만성자궁경염、자궁경첨예습우、자궁경린상상피CIN Ⅰ급、자궁경린상상피CIN Ⅲ급、자궁경침윤성린상세포암중적양성표체솔분별위:13.33%、90%、33.33%、0、0,기중첨예습우조HPV6/11적양성표체솔현저고우기타각조(균P＜0.001);CIN Ⅰ조여CIN Ⅲ급암비교차이유통계학의의(P=0.001,＜0.05);운용Spearman상관분석표명,HPV6/11재상술오충병변중적양성표체솔여악성도정부상관(rs=-0.370,P＜0.001).고위형HPV16/18재만성자궁경염、첨예습우、CIN Ⅰ급、CIN Ⅲ급、린상세포암중적양성표체솔분별위0、6.67%、10%、56.67%、76.67%,CIN Ⅲ급린암여기타각조간비교균유통계학의의(P＜0.001),단CIN Ⅲ여린암지간무통계학의의(P=0.10);운용Spearman상관분석표명,HPV16/18재상술오충병변중적양성표체솔여악성도정정상관(rs=0.628,P＜0.001).결론 저위형HPV6/11주요인기자궁경첨예습우급CIN Ⅰ;고위형HPV16/18여CIN Ⅲ급자궁경침윤성린암관계밀절,시인기자궁경CIN Ⅲ급침윤성암적주요인소.대HPV재자궁경병변중검측급분형유조우대자궁경병변적진단화감측,우기시대자궁경암적예방、조기진단급조기치료구유중요의의.
Objective We detected the expression of the low risk HPV6/11 and the high risk HPV16/18 in cervical lesions in order to investigate the correlativity,mechanism and clinical values between them.Methods We chosed 150 cases of cervical disease tissues that included chronic cervicitis (30 cases).cervieal condyloma without atypia(30 cases),CIN Ⅰ(30 cases),CIN Ⅲ(30 cases)and cervical squamous cell carcinoma (30 cases).And all of them were constructed into tissue microarrays(TMA)that include 300 spots.Then,the expression of HPV6/11 and HPV16/18 were determined by in situ hybridization.Results The positive expression rates of HPV6/11 were 13.33%,90%,33.33%,0 and 0 respectively in chronic cervicitis,cervical condyloma without atypia,CIN Ⅰ,CIN Ⅲ and cervical squamous cell carcinoma.Condyloma showed significantly higher HPV6/11 infection rates than the other groups(P＜0.001).And there were obvious dliference between CIN Ⅰ and CIN Ⅲ & carcinoma (P=0.001.＜0.05).The spearman correlation analysis suggested that the expression of HPV6/11 in the five cervical deseases was negative correlation (rs=-0.037,P＜0.001).The positive rates of HPV16/18 were 0,6.67%,10%.56.67%.76.67% respectively in chronic cervicitis,cervical condyloma without atypia,CIN Ⅰ,CIN Ⅲ and cervical squamous cell carcinoma.Comparing CIN Ⅲ and carcinoma with the other groups,the positive rates were obvious defrerence between them (P＜0.001).But there was not significant defference between CIN Ⅲ and carcinoma (P=0.10).The spearman correlation analysis suggested that the expression of HPV16/18 in the five cervical deseases was positive correlation (rs=0.628,P＜0.001).Conclusion Low risk HPV6/11 is a main reason for the cervical condyloma and CIN Ⅰ.High risk HPV16/18 is closely related with CIN Ⅲ and carsinoma.and also a main reason for CIN Ⅲ and carcinoma.Detecting and differentiating HPV type will help for the diagnosis and monitor of cervical lesions,especially,it will be very important to prophylaxis,early diagnosis and early therapy of cervical carcinoma.
