激光生物学报
激光生物學報
격광생물학보
ACTA LASER BIOLOGY SINICA
2007年
1期
6-11
,共6页
张楠%时永香%白增亮%张少军%李丽莉
張楠%時永香%白增亮%張少軍%李麗莉
장남%시영향%백증량%장소군%리려리
激光%蛋白质谱%双向电泳%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳%胚胎%中华大蟾蜍
激光%蛋白質譜%雙嚮電泳%十二烷基硫痠鈉-聚丙烯酰胺凝膠電泳%胚胎%中華大蟾蜍
격광%단백질보%쌍향전영%십이완기류산납-취병희선알응효전영%배태%중화대섬서
laser%patterns of protein%two dimensional electrophoresis%sodium dodecyl sylfate-polyacrylamide gel electrophresis ( SDS-PAGE )%embryo%Bufo bufo gargarizens
用YAG倍频激光器选择不同能量密度对中华大蟾蜍原肠胚进行照射,对各组样品进行双向电泳,凝胶成像后用PDQuest软件进行分析,结果表明:激光照射对蛋白质点数及其分布影响显著,影响程度与照射激光密度有关.各激光照射组与对照组相比,有些蛋白质点变化明显,有些蛋白质点在对照组未发现,而在各激光照射组皆新出现.结论:激光照射可对原肠胚一些蛋白质的表达产生明显的影响.
用YAG倍頻激光器選擇不同能量密度對中華大蟾蜍原腸胚進行照射,對各組樣品進行雙嚮電泳,凝膠成像後用PDQuest軟件進行分析,結果錶明:激光照射對蛋白質點數及其分佈影響顯著,影響程度與照射激光密度有關.各激光照射組與對照組相比,有些蛋白質點變化明顯,有些蛋白質點在對照組未髮現,而在各激光照射組皆新齣現.結論:激光照射可對原腸胚一些蛋白質的錶達產生明顯的影響.
용YAG배빈격광기선택불동능량밀도대중화대섬서원장배진행조사,대각조양품진행쌍향전영,응효성상후용PDQuest연건진행분석,결과표명:격광조사대단백질점수급기분포영향현저,영향정도여조사격광밀도유관.각격광조사조여대조조상비,유사단백질점변화명현,유사단백질점재대조조미발현,이재각격광조사조개신출현.결론:격광조사가대원장배일사단백질적표체산생명현적영향.
A YAG double-frequency laser microbeams was used to irradiate Bufo bufo gargarizens at the gastrula stage of its early development. The patterns of protein expression were investigated in gastrula by two-dimensional electrophoresis after irradiation with a laser. PDQuest software was used to examine the distribution of protein spots in two-dimensional gels after scanning with a GS-800 protein densitometer. The results demonstrated that protein expression was influenced by laser irradiation and the total number of protein spots was related to the intensity of the laser beam. There were some proteins obviously changed in laser treatment groups compared to the control and some spots visible in the treatment groups but not observed in the control.
