CAJ | 학술논문

通过人胚胎干细胞(human ernbryonie stem cells,hESC)体外分化方法和畸胎瘤形成可以分化获得多种成体细胞.但目前尚不清楚是否可以从hESCs畸胎瘤中分离某些特异性细胞.通过体外筛选方法,有效地从hESCs畸胎瘤中分离出神经前体细胞(neural progenitor cells,NPCs)和间充质干细胞(mesenchymal stem cells,MSCs).这种hESCs畸胎瘤来源的NPCs和MSCs与体内神经前体细胞和间充质干细胞有着相似的分子标记和特性,并具有进一步的分化潜能--分别可以诱导成为神经元、神经胶质细胞、脂肪细胞和骨骼细胞等.根据人胚胎干细胞畸胎瘤中含有不同分化阶段的外胚层、中胚层和内胚层的组织或细胞,认为人胚胎干细胞畸胎瘤可以作为另一个细胞来源以获取多种(包括人胚胎干细胞体外分化难以得到的)各种前体/干细胞和终末分化细胞.
통과인배태간세포(human ernbryonie stem cells,hESC)체외분화방법화기태류형성가이분화획득다충성체세포.단목전상불청초시부가이종hESCs기태류중분리모사특이성세포.통과체외사선방법,유효지종hESCs기태류중분리출신경전체세포(neural progenitor cells,NPCs)화간충질간세포(mesenchymal stem cells,MSCs).저충hESCs기태류래원적NPCs화MSCs여체내신경전체세포화간충질간세포유착상사적분자표기화특성,병구유진일보적분화잠능--분별가이유도성위신경원、신경효질세포、지방세포화골격세포등.근거인배태간세포기태류중함유불동분화계단적외배층、중배층화내배층적조직혹세포,인위인배태간세포기태류가이작위령일개세포래원이획취다충(포괄인배태간세포체외분화난이득도적)각충전체/간세포화종말분화세포.
Many somatic cell typos were obtained by in vitro differentiation or teratoma formation of human embryonic stem ceLls (hESCs). However, it is unclear whether specific cell types can be obtained from hESCs-derived teratoma. It was reported that many kinds of cells, including neural progetfitor/precursor cells (NPCs) and mesenchymal stem cells (MSCs) were isolated efficiently from the teratoma of hESCs through in vitro selection. The teratoma-derived NPCs and MSCs showed specific characteristics of molecular markers similar to the primary NPCs and MSCs. Moreover, these teratoma-induced NPCs and MSCs can be further induced to differentiate into neurons, astrocytes, or adipose and bone cells, reflecting their inherent multi-potencies. Given that teratoma normally contains a mixture of ectoderm, mesodenn, and endoderm lineage cells at different differentiation stage, it was suggested that hESCs-derived teratoma could be an alternative source to generate a variety of uncommitted progenitor cells or terminally differentiated somatic cells, which may be otherwise difficult to obtain through direct in vitro differentiation.