安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2009年
19期
9002-9005
,共4页
凌泽继%黎金秀%秦津%潘堂峰%宋小白%杨柄壮%许典新%韩涛%潘琼%李瑞明%谢莹雪%周辰瑜
凌澤繼%黎金秀%秦津%潘堂峰%宋小白%楊柄壯%許典新%韓濤%潘瓊%李瑞明%謝瑩雪%週辰瑜
릉택계%려금수%진진%반당봉%송소백%양병장%허전신%한도%반경%리서명%사형설%주신유
GnRH%下丘脑-垂体-卵巢轴%免疫组化%SuperPicTureTM二步法%摩杂一代水牛
GnRH%下丘腦-垂體-卵巢軸%免疫組化%SuperPicTureTM二步法%摩雜一代水牛
GnRH%하구뇌-수체-란소축%면역조화%SuperPicTureTM이보법%마잡일대수우
GnRH%Hypothalamus-pituitary-ovary axis%Immunohistochemistry%SuperPicTureTM two step method%Murrah hybrid water buffalo
[目的]研究促性腺激素释放激素(GnRH)在青年摩杂一代水牛下丘脑-垂体-卵巢轴的免疫组化定位.[方法]采用免疫组织化学SuperPicTureTM二步法检测5头青年摩杂一代水牛下丘脑、垂体和卵巢中GnRH的分布情况,研究GnRH在下丘脑、垂体及卵巢的分布上的联系,进而了解GnRH在下丘脑、垂体及卵巢三者之间分泌释放的途径.[结果]GnRH存在于青年摩杂一代水牛下丘脑-垂体-卵巢轴的各个环节.青年摩杂一代水牛下丘脑中各主要核团都有GnRH免疫阳性神经元的分布,尤其以视上核、视前核、室旁核分布较多.对于垂体,GnRH免疫阳性产物只存在于青年摩杂一代水牛的腺垂体中.青年摩杂一代水牛卵巢中生殖上皮细胞、颗粒细胞和黄体细胞发现有GnRH免疫阳性产物.[结论]为进一步研究GnRH在生殖中的生理和作用机制提供形态学依据,并对青年摩杂一代水牛的繁殖育种起到参考指导作用.
[目的]研究促性腺激素釋放激素(GnRH)在青年摩雜一代水牛下丘腦-垂體-卵巢軸的免疫組化定位.[方法]採用免疫組織化學SuperPicTureTM二步法檢測5頭青年摩雜一代水牛下丘腦、垂體和卵巢中GnRH的分佈情況,研究GnRH在下丘腦、垂體及卵巢的分佈上的聯繫,進而瞭解GnRH在下丘腦、垂體及卵巢三者之間分泌釋放的途徑.[結果]GnRH存在于青年摩雜一代水牛下丘腦-垂體-卵巢軸的各箇環節.青年摩雜一代水牛下丘腦中各主要覈糰都有GnRH免疫暘性神經元的分佈,尤其以視上覈、視前覈、室徬覈分佈較多.對于垂體,GnRH免疫暘性產物隻存在于青年摩雜一代水牛的腺垂體中.青年摩雜一代水牛卵巢中生殖上皮細胞、顆粒細胞和黃體細胞髮現有GnRH免疫暘性產物.[結論]為進一步研究GnRH在生殖中的生理和作用機製提供形態學依據,併對青年摩雜一代水牛的繁殖育種起到參攷指導作用.
[목적]연구촉성선격소석방격소(GnRH)재청년마잡일대수우하구뇌-수체-란소축적면역조화정위.[방법]채용면역조직화학SuperPicTureTM이보법검측5두청년마잡일대수우하구뇌、수체화란소중GnRH적분포정황,연구GnRH재하구뇌、수체급란소적분포상적련계,진이료해GnRH재하구뇌、수체급란소삼자지간분비석방적도경.[결과]GnRH존재우청년마잡일대수우하구뇌-수체-란소축적각개배절.청년마잡일대수우하구뇌중각주요핵단도유GnRH면역양성신경원적분포,우기이시상핵、시전핵、실방핵분포교다.대우수체,GnRH면역양성산물지존재우청년마잡일대수우적선수체중.청년마잡일대수우란소중생식상피세포、과립세포화황체세포발현유GnRH면역양성산물.[결론]위진일보연구GnRH재생식중적생리화작용궤제제공형태학의거,병대청년마잡일대수우적번식육충기도삼고지도작용.
[Objective] The research aimed to study the distribution of gonadotropin (GnRH) releasing hormone in hypothalamus-pituitary-ovary axis of youth murrah hybrid water buffalo.[Method] The immunohistochemistry SuperPicTureTM two step method was used to detect the distribution of GnRH in hypothalamus-pituitary-ovary axis of five femal murrah hybrid water buffalos.The association of GnRH 's distribution in hypothalamus, pituitary and ovary, and understand how the GnRH secrete releasing in hypothalamus, pituitary and ovary.[Result] The GnRH was first discovered in singulorum link of hypothalamus-pituitary-ovary axis of murrah hybrid water bufflo.The GnRH immunoreactive neurons were first discovered in every main nucleus of murrah hybrid water bufflo hypothalamus, especially distributed in supraoptic nucleus, preoptic nucleus, paraventricular nucleus most.The GnRH immunoactive productions were first discovered only in murrah hybrid water bufflo adenohypophysis for pituitary.The GnRH immunoactive productions were first discovered in the germinal epithelium cells, granulosa cells and lutein cells of murrah hybrid water bufflo.[Conclusion] The test results could provide morphological basis for the study of the GnRH's synthesis and mechanism of action, and could play reference and directions part in breeding murrah hybrid water bufflo.
