CAJ | 학술논문

糖链结构的质谱解析是今后糖蛋白分析中的重要研究内容,其中完整糖肽的分析,由于可以同时获得糖基化位点和对应糖链的结构信息,更具有重要意义和研究前景.本工作对质谱软电离技术在完整糖肽分析中的应用进行了研究,其中包括了基质辅助激光解吸电离(matrix-assisted laser desorption ionization,MALDI)和电喷雾电离(electrospray ionization,ESI)技术.通过平行使用两种串联质谱(tandem mass spectrometry,MS/MS)分析策略:MALDI-MS/MS和ESI-MS/MS对目标糖蛋白--辣根过氧化物酶进行分析,并讨论了其互补性.结果表明,MALDI和ESI技术各有优劣,结合串联质谱分析,可获得糖肽的糖链结构信息;两条路线互补使用,在揭示蛋白质糖基化修饰(位点和结构)的研究中十分必要.
당련결구적질보해석시금후당단백분석중적중요연구내용,기중완정당태적분석,유우가이동시획득당기화위점화대응당련적결구신식,경구유중요의의화연구전경.본공작대질보연전리기술재완정당태분석중적응용진행료연구,기중포괄료기질보조격광해흡전리(matrix-assisted laser desorption ionization,MALDI)화전분무전리(electrospray ionization,ESI)기술.통과평행사용량충천련질보(tandem mass spectrometry,MS/MS)분석책략:MALDI-MS/MS화ESI-MS/MS대목표당단백--랄근과양화물매진행분석,병토론료기호보성.결과표명,MALDI화ESI기술각유우렬,결합천련질보분석,가획득당태적당련결구신식;량조로선호보사용,재게시단백질당기화수식(위점화결구)적연구중십분필요.
The mass spectrometric analysis of glycan composition and structure is a difficult but essential part in future study following the present glycoprotein identification.Intact glycopeptides analysis is an attracting field,in considering its capability to provide glycosite and corresponding glycan structure information at the same time.Mass spectrometry has been proven to be an important and a key tool for glycan analysis over the past few years.Making use of two soft ionization techniques-matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI),through tandem mass spectrometry (MS/MS) analysis,the information for glycans of the glycopeptides can be obtained in the investigation.Meanwhile,by comparing the MALDI-MS/MS and ESI-MS/MS approaches using model glycoprotein (horseradish peroxidase,HRP),the complementary results have been verified experimentally.We believe that the combination of the two techniques is necessary and will provide useful information for the understanding of protein glycosylation.