CAJ | 학술논문

建立腊肠中罗丹明B(rhodamine B)残留量的高效液相色谱串联质谱榆测方法.试样中残留的罗丹明B用乙酸乙酯-环己烷(1:1,V/V)提取,经凝胶色谱净化系统净化并浓缩后,用液相色谱-质谱/质谱仪测定,外标峰面积法定量.罗丹明B在5～100ng/mL范围内呈良好的线性关系,3个水平(5.0、10.0、25.0 μg/kg)添加罗丹明B的回收率为79.8%～110.3%,相对标准偏差(RSD)为10.O%～10.3%,检出限为5 μg/kg.本方法简单、快速,适用于腊制品中残留罗丹明B的检测.
건립석장중라단명B(rhodamine B)잔류량적고효액상색보천련질보유측방법.시양중잔류적라단명B용을산을지-배기완(1:1,V/V)제취,경응효색보정화계통정화병농축후,용액상색보-질보/질보의측정,외표봉면적법정량.라단명B재5～100ng/mL범위내정량호적선성관계,3개수평(5.0、10.0、25.0 μg/kg)첨가라단명B적회수솔위79.8%～110.3%,상대표준편차(RSD)위10.O%～10.3%,검출한위5 μg/kg.본방법간단、쾌속,괄용우석제품중잔류라단명B적검측.
A high-performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS)method was developed for the determination of rhodamine B residue in Chinese preserved sausages.The whole determination procedure consisted of extraction with a mixture of ethyl acetate and cyclohexane(1:1,V/V),cleanup with an Aecuprep gel filtration chromatographic system,rotary evaporation concentration,filtration through 0.22 μm membrane,HPLC-MS/MS analysis,and quantitation by external standard method.The developed method showed a good linesrity over the wide range of 5-100 ng/mL.Average recoveries of rhodamine B in spiked samples at three levels varied from 79.8% to 110.3%,with relative standard deviations between 10.0% and 10.3%.The limit of detection was 5 μg/kg.Therefore,this method,thanks to its simplicity and rapidity,has a good applicability to the determination of rhodamine B residue in Chinese preserved sausages.