CAJ | 학술논문

背景:研究表明环氧化酶2和基质金属蛋白酶2在角膜新生血管的发生中起重要作用,但其具体机制及相互关系仍不明确.目的:观察环氧化酶2和基质金属蛋白酶2在缝线法诱导的大鼠角膜新生血管中的表达,并分析其相关性,探讨角膜新生血管形成的有关机制.方法:采用缝线法建立SD大鼠角膜新生血管模型,术后每日裂隙灯观察角膜新生血管的生长情况,并于1,4,7,14,21 d取材,行免疫组化及RT-PCR检测环氧化酶2和基质金属蛋白酶2蛋白的分布及二者mRNA的相对表达量,并进行相关性分析.结果与结论:角膜缝线后三四天可见明显从角膜缘伸入角膜的毛刷状小血管,垂直角膜缘切线方向,角膜缝线处水肿;7 d角膜新生血管生长旺盛,角膜水肿继续加重,14 d新生血管延伸到达或超过缝线位置,分支密集并互相吻合形成袢状血管;21 d角膜新生血管变细.免疫组化及RT-PCR显示环氧化酶2和基质金属蛋白酶2于缝线后表达逐渐增加,7 d达高峰,以后随炎症细胞的减少而减弱,主要分布于炎症细胞胞质及新生血管内皮细胞,两种因子的表达在角膜新生血管中具有正相关性(r=0.981,P<0.05).证实炎症相关的角膜新生血管中环氧化酶2和基质金属蛋白酶2的表达增加,并且与新生血管的发生、发展密切相关.
배경:연구표명배양화매2화기질금속단백매2재각막신생혈관적발생중기중요작용,단기구체궤제급상호관계잉불명학.목적:관찰배양화매2화기질금속단백매2재봉선법유도적대서각막신생혈관중적표체,병분석기상관성,탐토각막신생혈관형성적유관궤제.방법:채용봉선법건립SD대서각막신생혈관모형,술후매일렬극등관찰각막신생혈관적생장정황,병우1,4,7,14,21 d취재,행면역조화급RT-PCR검측배양화매2화기질금속단백매2단백적분포급이자mRNA적상대표체량,병진행상관성분석.결과여결론:각막봉선후삼사천가견명현종각막연신입각막적모쇄상소혈관,수직각막연절선방향,각막봉선처수종;7 d각막신생혈관생장왕성,각막수종계속가중,14 d신생혈관연신도체혹초과봉선위치,분지밀집병호상문합형성번상혈관;21 d각막신생혈관변세.면역조화급RT-PCR현시배양화매2화기질금속단백매2우봉선후표체축점증가,7 d체고봉,이후수염증세포적감소이감약,주요분포우염증세포포질급신생혈관내피세포,량충인자적표체재각막신생혈관중구유정상관성(r=0.981,P<0.05).증실염증상관적각막신생혈관중배양화매2화기질금속단백매2적표체증가,병차여신생혈관적발생、발전밀절상관.
BACKGROUND:Studies has demonstrated that cyclooxygenase-2(COX-2)and matfix metelloproteinase-2(MMP-2) may play an important role in corneal neovasculadzation(CNV),but their mechanisms and interactions Fernain unclear.OBJECTIVE:To investigate the expressions of COX-2 and MMP-2 in suture-induced CNV,and to analyze the interaction between COX-2 and MMP-2,additionally.to explore the mechanisms of CNV.METHODS:The models of CNV were induced by comeal sUtch.The development of CNV was monitored daily under slit-lamp microscope.The expressions of COX-2 and MMP-2 and levels of COX-2 and MMP-2 mRNA in CNV were detected by immunohistochemistry and reverse transcription polymerase chain reaction(RT-PCR)at days 1,4,7,14 and 21 after operation,and their correlations were analyzed.RESULTS AND CONCLUSION:New vessels appeared near corneal limbus and grew towards into transparent cornea at 3-4 days after stitching.The stitch sites were edematous.The neovascular thdved and edema increased at 7 days.The neovascular reached to stitch sites at 14 days,and then vascular net formed.Then vessels gradually became thinner at 21 days.Immunochemistry and RT-PCR demonstrated:the positive expressions of COX-2 and MMP-2 in CNV localized in inflammatory cells and endothelial cells of neovasculadzation progressed gradually after stitching,and involved the entire cornea at the 7 day post-treatment.After 7 days the expression decreased following the decrease of inflammatory cells.The expression of COX-2 was correlative with MMP-2 positively(r=0.981,P<0.05).All results demonstrated that in inflammation-associated corneal angiogenesis,expression of COX-2 and MMP-2 progressed and closely relaled to the angiogenesis.