食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
6期
220-222
,共3页
胡君萍%杨建华%王新玲%木艾塔尔·努尔麦麦提%热娜·卡斯木
鬍君萍%楊建華%王新玲%木艾塔爾·努爾麥麥提%熱娜·卡斯木
호군평%양건화%왕신령%목애탑이·노이맥맥제%열나·잡사목
沙枣果实%总酚%Folin-Ciocalteu比色法
沙棘果實%總酚%Folin-Ciocalteu比色法
사조과실%총분%Folin-Ciocalteu비색법
Elaeagnus angustifolia fruit%total polyphenols%Folin-Ciocalteu colorimetry
以没食子酸为对照品,采用Folin-Ciocalteu比色法测定新疆不同产地沙枣果肉及种子中总酚含量.没食子酸在2~12μg/mL范围内与吸光度呈良好线性关系(r=0.9991),平均回收率为98.80%,RSD为1.81%.7个产地沙枣种子中的总酚含最均明显高于果肉,种子总酚含量/果肉总酚含量约为3~5倍.实验方法简便、快速、准确,可用于沙枣果实的质量控制.
以沒食子痠為對照品,採用Folin-Ciocalteu比色法測定新疆不同產地沙棘果肉及種子中總酚含量.沒食子痠在2~12μg/mL範圍內與吸光度呈良好線性關繫(r=0.9991),平均迴收率為98.80%,RSD為1.81%.7箇產地沙棘種子中的總酚含最均明顯高于果肉,種子總酚含量/果肉總酚含量約為3~5倍.實驗方法簡便、快速、準確,可用于沙棘果實的質量控製.
이몰식자산위대조품,채용Folin-Ciocalteu비색법측정신강불동산지사조과육급충자중총분함량.몰식자산재2~12μg/mL범위내여흡광도정량호선성관계(r=0.9991),평균회수솔위98.80%,RSD위1.81%.7개산지사조충자중적총분함최균명현고우과육,충자총분함량/과육총분함량약위3~5배.실험방법간편、쾌속、준학,가용우사조과실적질량공제.
Gallic acid was used as a standard substance to determine total polyphenol contents in different parts of Elaeagnus angustifolia fruit growing in Xinjiang Autonomous Region by Folin-Ciocalteu method.The calibration curve of gallic acid presented good linearity within the concentration range of 2-12 μg/mL (r = 0.9991).The average of three replicates of spike recovery for total polyphenols at three levels was 98.80%,with a relative standard deviation of 1.81%.Elaeagnus angustifolia seeds exhibited significantly higher total polyphenol content than Elaeagnus angustifolia flesh,with a 3-to 5-fold increase.The method demonstrates the advantages of convenience,rapidity and accuracy,thereby providing an effective approach for the quality control of Elaeagnus angustifolia fruit.