宜春学院学报
宜春學院學報
의춘학원학보
JOURNAL OF YICHUN UNIVERSITY
2011年
8期
74-76
,共3页
赵启铎%舒乐新%马琳%崔国红
趙啟鐸%舒樂新%馬琳%崔國紅
조계탁%서악신%마림%최국홍
槐甘菌质%多糖%硫酸-苯酚法%含量测定
槐甘菌質%多糖%硫痠-苯酚法%含量測定
괴감균질%다당%류산-분분법%함량측정
Huaiganjunzhi%polysaccharide%phenol-sulfuric acid method%content determination
目的：建立槐甘菌质多糖的含量测定方法。方法：硫酸-苯酚法测定槐甘菌质多糖的含量。结果：葡萄糖质量浓度在10.08～80.64mg.L-1时与吸光度有良好的线性关系,线性方程为Y=0.018X＋0.0397（r=0.9995）,加样回收率分别为99.86%（80%,n=3）,RSD 0.29%;103.11%（100%,n=3）,RSD 0.41%;103.10%（120%,n=3）,RSD 0.81%。结论：该方法简便、稳定性和重复性好、结果可靠,可用于测定槐甘菌质多糖含量。
目的：建立槐甘菌質多糖的含量測定方法。方法：硫痠-苯酚法測定槐甘菌質多糖的含量。結果：葡萄糖質量濃度在10.08～80.64mg.L-1時與吸光度有良好的線性關繫,線性方程為Y=0.018X＋0.0397（r=0.9995）,加樣迴收率分彆為99.86%（80%,n=3）,RSD 0.29%;103.11%（100%,n=3）,RSD 0.41%;103.10%（120%,n=3）,RSD 0.81%。結論：該方法簡便、穩定性和重複性好、結果可靠,可用于測定槐甘菌質多糖含量。
목적：건립괴감균질다당적함량측정방법。방법：류산-분분법측정괴감균질다당적함량。결과：포도당질량농도재10.08～80.64mg.L-1시여흡광도유량호적선성관계,선성방정위Y=0.018X＋0.0397（r=0.9995）,가양회수솔분별위99.86%（80%,n=3）,RSD 0.29%;103.11%（100%,n=3）,RSD 0.41%;103.10%（120%,n=3）,RSD 0.81%。결론：해방법간편、은정성화중복성호、결과가고,가용우측정괴감균질다당함량。
Objective： To establish a method for the determination of polysaccharide in huaiganjunzhi.method： The content of polysaccharide was determined by method of phenol-sulfuric acid.Results： In the range of 10.08~80.64mg·L-1,glucose concentration showed a good linearity with absorbance values,the linear equation being Y= 0.018X ＋ 0.0397（r = 0.9995）,The average recovery rate was 99.86%（80%,n =3）,RSD was 0.29%;103.11%（100%,n =3）,RSD was 0.41%;103.10%（120%,n =3）,RSD was 0.81%,respectively.Conclusion： The method was proved to be simple,accurate with good stability and reproducibility,and can be used to determine the polysaccharide in Huaiganjunzhi.
