CAJ | 학술논문

目的探讨面神经损伤动物模型中CD4+ T细胞与损伤的面运动神经元(facial motoneuron,FMN)之间的关系,为揭示面神经损伤后修复及再生中的免疫病理机制提供实验证据.方法磁珠分选法分离野生型BALB/c小鼠CD4+ T细胞,经尾静脉移植于相同遗传背景的裸小鼠,并以PBS为对照.1周后均制备成周围性面神经损伤模型,指定时间在面神经断端涂抹荧光金示踪剂.术后3 d灌注固定动物,收集脑于切片,荧光显微镜下观察.采用Image Pro Plus 5.1图像分析软件计数FMN,并比较不同组之间的差异.结果术后3 d CD4+ T细胞移植组FMN计数为3444.5±84.2(-x±s,下同),对照组FMN计数为3013.2±65.3,差异具有统计学意义(t=5.52,P=0.0003);而CD4+ T细胞移植组与野生型小鼠神经元计数比较,差异无统计学意义(t=0.49,P=0.6347).结论 CD4+ T细胞可以保护裸鼠受损伤的FMN,使其数量达到野生型的水平.
목적 탐토면신경손상동물모형중CD4+ T세포여손상적면운동신경원(facial motoneuron,FMN)지간적관계,위게시면신경손상후수복급재생중적면역병리궤제제공실험증거.방법 자주분선법분리야생형BALB/c소서CD4+ T세포,경미정맥이식우상동유전배경적라소서,병이PBS위대조.1주후균제비성주위성면신경손상모형,지정시간재면신경단단도말형광금시종제.술후3 d관주고정동물,수집뇌우절편,형광현미경하관찰.채용Image Pro Plus 5.1도상분석연건계수FMN,병비교불동조지간적차이.결과 술후3 d CD4+ T세포이식조FMN계수위3444.5±84.2(-x±s,하동),대조조FMN계수위3013.2±65.3,차이구유통계학의의(t=5.52,P=0.0003);이CD4+ T세포이식조여야생형소서신경원계수비교,차이무통계학의의(t=0.49,P=0.6347).결론 CD4+ T세포가이보호라서수손상적FMN,사기수량체도야생형적수평.
Objective To investigate the specific T cell subpopulation and the relationship with facial motoneuron in immune deficiency mouse model with facial nerve paralysis, so as to find information for new strategy of facial palsy treatment. Methods Firstly, purifying the CD4+ T cell from wild type mouse and reestablishing the immune function of nude mouse by infusing the CD4+ T cell through the tail vein a week before the surgery. Then the all nude mouse(BALB/c background)and wild type mouse(BALB/c background)were subjected to a right facial nerve axotomy. Then the mouse was studied by application and assessment with fluorogold retrotraeer at specific time. After collecting the slices of brain stem three days post the operation,the facial motoneurons was observed under fluorescence microscope, then analyzed and counted with the software Image Pro Plus5.1. Results The number of survival facial motoneuron in the group with CD4+ T cell transplantation and control group was(3444.5±84.2,-x±s)and(3013.2±65.3)respectively. There was significant difference of the number of survival facial motoneurons between nude mouse transplanted with CD4+ T cell and PBS at three days post the operation(t=5.52,P=0.0003).But there was no significant difference of survival facial motoneurons between nude mouse transplanted with CD4+ T cell and wild type mouse three days post the operation(t=0.49,P=0.6347). It was the transplantation of CD4+ T cell that rescued the survival facial motoneuron to the level of wild type.Conclusions CD4+ T cell have the ability to rescue the iniuring facial motoneuron from death. It may suggest that there is a critical role of the specific T cell subpopulation in facial nerve repair and regeneration.