中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2011年
1期
49-52
,共4页
靳昌忠%冯磊%谢天胜%吴丽娟%方美新%张福杰%赵燕%吴南屏
靳昌忠%馮磊%謝天勝%吳麗娟%方美新%張福傑%趙燕%吳南屏
근창충%풍뢰%사천성%오려연%방미신%장복걸%조연%오남병
儿童%获得性免疫缺陷综合征%抗逆转录病毒治疗,高效%CD8阳性T淋巴细胞%抗原,CD38%HLA-DR抗原
兒童%穫得性免疫缺陷綜閤徵%抗逆轉錄病毒治療,高效%CD8暘性T淋巴細胞%抗原,CD38%HLA-DR抗原
인동%획득성면역결함종합정%항역전록병독치료,고효%CD8양성T림파세포%항원,CD38%HLA-DR항원
Child%Acquired immunodeficiency syndrome%Antiretroviral therapy,highly active%CD8-positive T-lymphocytes%Antigens,CD38%HLA-DR antigens
目的 观察高效抗逆转录病毒治疗(HAART)对我国艾滋病患儿CD8+T细胞活化标志分子CD38和人类白细胞抗原DR(HLA-DR)表达水平的影响及其与病毒载量的关系.方法 对194例接受HAART的艾滋病患儿进行横断面研究,用流式细胞术检测CD4+、CD8+T细胞数,以及CD8+/CD38+和CD8+/HLA-DR+T细胞比例,RT-PCR检测血浆HIV RNA载量.并检测52名健康儿童CD8+/CD38+和CD8+/HLA-DR+T细胞比例作为正常对照.结果 本组中,135例病毒载量＜400 copies/ml,59例病毒载量≥400 copies/ml.病毒载量≥400 copies/ml组CD8+/CD38+T和CD8+/HLA-DR+T细胞水平显著高于病毒载量＜400 copies/ml组,差异有统计学意义(29.6±10.1 vs19.9±9.8;17.7±6.4 vs 9.6±6.1,P＜0.05);病毒载量＜400 copies/ml组,CD8+/CD38+T细胞接近正常水平,而CD8+/HLA-DR+T细胞仍高于正常水平,差异有统计学意义(19.9±9.8 vs 15.6±9.0;9.6±6.1 vs 5.8±3.3,P＜0.05).CD8+/CD38+和CD8+/HLA-DR+T细胞百分比均与病毒载量成正相关(前者相关系数R=0.403,P=0.03,后者相关系数R=0.569,P=0.09).结论 艾滋病患儿CD8+T细胞活化程度与病毒载量成正比,有效的HAART治疗能够显著地降低HIV感染者免疫活化程度,CD8+/CD38+和CD8+/HLA-DR+T细胞百分比可能是资源有限地区替代病毒载量检测的潜在指标.
目的 觀察高效抗逆轉錄病毒治療(HAART)對我國艾滋病患兒CD8+T細胞活化標誌分子CD38和人類白細胞抗原DR(HLA-DR)錶達水平的影響及其與病毒載量的關繫.方法 對194例接受HAART的艾滋病患兒進行橫斷麵研究,用流式細胞術檢測CD4+、CD8+T細胞數,以及CD8+/CD38+和CD8+/HLA-DR+T細胞比例,RT-PCR檢測血漿HIV RNA載量.併檢測52名健康兒童CD8+/CD38+和CD8+/HLA-DR+T細胞比例作為正常對照.結果 本組中,135例病毒載量＜400 copies/ml,59例病毒載量≥400 copies/ml.病毒載量≥400 copies/ml組CD8+/CD38+T和CD8+/HLA-DR+T細胞水平顯著高于病毒載量＜400 copies/ml組,差異有統計學意義(29.6±10.1 vs19.9±9.8;17.7±6.4 vs 9.6±6.1,P＜0.05);病毒載量＜400 copies/ml組,CD8+/CD38+T細胞接近正常水平,而CD8+/HLA-DR+T細胞仍高于正常水平,差異有統計學意義(19.9±9.8 vs 15.6±9.0;9.6±6.1 vs 5.8±3.3,P＜0.05).CD8+/CD38+和CD8+/HLA-DR+T細胞百分比均與病毒載量成正相關(前者相關繫數R=0.403,P=0.03,後者相關繫數R=0.569,P=0.09).結論 艾滋病患兒CD8+T細胞活化程度與病毒載量成正比,有效的HAART治療能夠顯著地降低HIV感染者免疫活化程度,CD8+/CD38+和CD8+/HLA-DR+T細胞百分比可能是資源有限地區替代病毒載量檢測的潛在指標.
목적 관찰고효항역전록병독치료(HAART)대아국애자병환인CD8+T세포활화표지분자CD38화인류백세포항원DR(HLA-DR)표체수평적영향급기여병독재량적관계.방법 대194례접수HAART적애자병환인진행횡단면연구,용류식세포술검측CD4+、CD8+T세포수,이급CD8+/CD38+화CD8+/HLA-DR+T세포비례,RT-PCR검측혈장HIV RNA재량.병검측52명건강인동CD8+/CD38+화CD8+/HLA-DR+T세포비례작위정상대조.결과 본조중,135례병독재량＜400 copies/ml,59례병독재량≥400 copies/ml.병독재량≥400 copies/ml조CD8+/CD38+T화CD8+/HLA-DR+T세포수평현저고우병독재량＜400 copies/ml조,차이유통계학의의(29.6±10.1 vs19.9±9.8;17.7±6.4 vs 9.6±6.1,P＜0.05);병독재량＜400 copies/ml조,CD8+/CD38+T세포접근정상수평,이CD8+/HLA-DR+T세포잉고우정상수평,차이유통계학의의(19.9±9.8 vs 15.6±9.0;9.6±6.1 vs 5.8±3.3,P＜0.05).CD8+/CD38+화CD8+/HLA-DR+T세포백분비균여병독재량성정상관(전자상관계수R=0.403,P=0.03,후자상관계수R=0.569,P=0.09).결론 애자병환인CD8+T세포활화정도여병독재량성정비,유효적HAART치료능구현저지강저HIV감염자면역활화정도,CD8+/CD38+화CD8+/HLA-DR+T세포백분비가능시자원유한지구체대병독재량검측적잠재지표.
Objective To study the expression of CD38 and HLA-DR on CD8 + T cells in pediatric AIDS patients receiving highly active antiretroviral therapy (HAART) and the relationship of immune activation and disease progression. Methods A cross-section study of 194 pediatric AIDS patients receiving HAART was carried out and 52 age-matched healthy children were recruited as control. The percentage of CD4+ , CD8+ , CD8+/CD38 + and CD8+/HLA-DR+ T cells was tested using flow cytometry, and HIVRNA in plasma was detected by quantitative RT-PCR. Results One hundred and ninety-four pediatric AIDS patients were divided into two groups according to the viral load: 59 patients with VL≥400 copies/ml and 135 patients with VL＜400 copies/ml. The percentage of CD8 +/CD38+ and CD8 +/HLA-DR+ T cells of patients with VL≥400 copies/ml was significantly higher than that of patients with VL ＜ 400 copies/ml (P ＜ 0. 05 ). Of patients with VL ＜ 400 copies/ml, the percentage of CD8 +/CD38 + T cells was nearly normal, and the percentage of CD8 +/HLA-DR+ T cells was higher than normal level ( P ＜ 0. 05 ). There was a positive correlation between percentage of CD8+/CD38+ and of CD8 +/HLA-DR+ T cells and viral load ( R = 0. 403, P = 0. 03 for the former and R = 0. 569, P = 0. 09 for the later). Conclusions Effective HAART could decrease immune activation of HIV-infected children significantly. And there was a positive correlation between percentage of CD8 +/CD38 + and of CD8 +/HLA-DR + T cells and viral load, suggesting that the two indicators might be used as the substitution of viral load in resource-limited areas.
