中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2008年
6期
346-349
,共4页
林三福%宋建榕%林明锋%莫家栋%李建东%陈敏
林三福%宋建榕%林明鋒%莫傢棟%李建東%陳敏
림삼복%송건용%림명봉%막가동%리건동%진민
骨化三醇%免疫抑制剂%骨移植%T淋巴细胞
骨化三醇%免疫抑製劑%骨移植%T淋巴細胞
골화삼순%면역억제제%골이식%T림파세포
Calcitriol%Immunosuppressive agents%Bone transplantation%T lymphocyte
目的 探讨应用骨化三醇(1,25-二羟维生素D;简称:VD5)在抑制肢体移植排斥反应中的作用.方法 以Wistar大鼠为供者,SD大鼠为受者,建立同种肢体移植模型.随机将受者分为4组,每组12只.(1)对照组:术后不用免疫抑制剂,仅以15 ml·kg-1·d-1.生理盐水灌服.(2)他克莫司(FK506)组:将FK506用生理盐水稀释为0.5 mg/ml,术后前2周的用量为1.0 mg·kg-1·d-1,术后第3周起,每周灌服2次.(3)VD3组:将骨化三醇用生理盐水稀释为0.125 μg/ml,术后用量为2.5μg·kg-1·d-1,连续灌服4周.(4)VD3+FK506组:术后联合应用骨化三醇及FK506,用药方法和用量与FK506组和VD3组相同.术后观察各组受者移植肢体排斥反应发生的时间和存活时间;流式细胞仪检测T淋巴细胞亚群CD4+、CD8+细胞的百分率.结果 对照组、FK506组、VD3组以及VD3+FK506组肢体移植后排斥反应发生的时间分别为:(3.50±0.50)d、(13.13±1.50)d、(10.63±0.38)d和(29.25±0.63)d;移植肢体的存活时间分别为:(8.50±0.50)d、(26.25±1.50)d、(17.25±0.38)d和(62.00±0.63)d;与对照组比较,VD3组排斥反应发生的时间和移植肢体的存活时间均明显延长,差异有统计学意义(P＜0.01);VD3+FK506组抗排斥反应效果更佳,与FK506组和VD3组比较,差异有统计学意义(P＜0.01).VD3组T淋巴细胞亚群CD4+/CD8+细胞的比值明显低于对照组,VD3+FK506组又明显低于VD3组和FK506组(P＜0.01).结论 骨化三醇能明显减轻同种肢体移植排斥反应,延长移植肢体的存活时间;骨化三醇与FK506联合应用效果更佳,二者具有协同作用.
目的 探討應用骨化三醇(1,25-二羥維生素D;簡稱:VD5)在抑製肢體移植排斥反應中的作用.方法 以Wistar大鼠為供者,SD大鼠為受者,建立同種肢體移植模型.隨機將受者分為4組,每組12隻.(1)對照組:術後不用免疫抑製劑,僅以15 ml·kg-1·d-1.生理鹽水灌服.(2)他剋莫司(FK506)組:將FK506用生理鹽水稀釋為0.5 mg/ml,術後前2週的用量為1.0 mg·kg-1·d-1,術後第3週起,每週灌服2次.(3)VD3組:將骨化三醇用生理鹽水稀釋為0.125 μg/ml,術後用量為2.5μg·kg-1·d-1,連續灌服4週.(4)VD3+FK506組:術後聯閤應用骨化三醇及FK506,用藥方法和用量與FK506組和VD3組相同.術後觀察各組受者移植肢體排斥反應髮生的時間和存活時間;流式細胞儀檢測T淋巴細胞亞群CD4+、CD8+細胞的百分率.結果 對照組、FK506組、VD3組以及VD3+FK506組肢體移植後排斥反應髮生的時間分彆為:(3.50±0.50)d、(13.13±1.50)d、(10.63±0.38)d和(29.25±0.63)d;移植肢體的存活時間分彆為:(8.50±0.50)d、(26.25±1.50)d、(17.25±0.38)d和(62.00±0.63)d;與對照組比較,VD3組排斥反應髮生的時間和移植肢體的存活時間均明顯延長,差異有統計學意義(P＜0.01);VD3+FK506組抗排斥反應效果更佳,與FK506組和VD3組比較,差異有統計學意義(P＜0.01).VD3組T淋巴細胞亞群CD4+/CD8+細胞的比值明顯低于對照組,VD3+FK506組又明顯低于VD3組和FK506組(P＜0.01).結論 骨化三醇能明顯減輕同種肢體移植排斥反應,延長移植肢體的存活時間;骨化三醇與FK506聯閤應用效果更佳,二者具有協同作用.
목적 탐토응용골화삼순(1,25-이간유생소D;간칭:VD5)재억제지체이식배척반응중적작용.방법 이Wistar대서위공자,SD대서위수자,건립동충지체이식모형.수궤장수자분위4조,매조12지.(1)대조조:술후불용면역억제제,부이15 ml·kg-1·d-1.생리염수관복.(2)타극막사(FK506)조:장FK506용생리염수희석위0.5 mg/ml,술후전2주적용량위1.0 mg·kg-1·d-1,술후제3주기,매주관복2차.(3)VD3조:장골화삼순용생리염수희석위0.125 μg/ml,술후용량위2.5μg·kg-1·d-1,련속관복4주.(4)VD3+FK506조:술후연합응용골화삼순급FK506,용약방법화용량여FK506조화VD3조상동.술후관찰각조수자이식지체배척반응발생적시간화존활시간;류식세포의검측T림파세포아군CD4+、CD8+세포적백분솔.결과 대조조、FK506조、VD3조이급VD3+FK506조지체이식후배척반응발생적시간분별위:(3.50±0.50)d、(13.13±1.50)d、(10.63±0.38)d화(29.25±0.63)d;이식지체적존활시간분별위:(8.50±0.50)d、(26.25±1.50)d、(17.25±0.38)d화(62.00±0.63)d;여대조조비교,VD3조배척반응발생적시간화이식지체적존활시간균명현연장,차이유통계학의의(P＜0.01);VD3+FK506조항배척반응효과경가,여FK506조화VD3조비교,차이유통계학의의(P＜0.01).VD3조T림파세포아군CD4+/CD8+세포적비치명현저우대조조,VD3+FK506조우명현저우VD3조화FK506조(P＜0.01).결론 골화삼순능명현감경동충지체이식배척반응,연장이식지체적존활시간;골화삼순여FK506연합응용효과경가,이자구유협동작용.
Objective To investigate the effects of Calcitriol (1, 25-dihydroxyvitamin D3, VD3) on rejection of allogenic limb transplantation. Methods Rat allogenic limb transplantation model (Wistar rats→ SD rats) was established. Recipients were divided into 4 groups randomly: 12 recipients received normal saline solution (15 nag·ml-1·d-1 orally) (group A); 12 recipients received FK506 solution (1.0 mg·ml-1·d-1) for 2 weeks and then 1.0 mg/ml twice a week (group B); 12 recipients received Calcitriol solution (2.5 μg-1ml-1·d-1) for 4 weeks (group C); 12 recipients received FK506 solution and Caleitriol solution (group D). Mean survival time (MST) of the limb grafts and mean time of rejection were observed. Flow cytometry was used to detect the expression of CD4+ and CD8+ in T lymphocytes. Results Mean time of rejection was (3.50±0.50) d in group A, (13.13±1.50) d in group B, (10.63±0.38) d in group C and (29.25±0.63) d in group D. MST of the limb grafts in groups A, B, C and D was (8.50±0.50)d, (26.25±1.50)d, (17.25±0.38)d and (62.00±0.63)d, respectively. There was significant difference between group A and group C in MST of the limb grafts and mean time of rejection (P＜0.01). Significant differences were also observed between group D and groups B, group C in these 2 indexes (P＜0.01). The percentage of CD4+ and CD8+ T lymphocytes in group C was less than that in group A (P＜0.01), and that in group D was less than that in group B and group C (P＜0.01). Conclusions Caldtriol can reduce the occurrence of reaction of allogenie limb transplantation and prolong MST of the grafts. Because of synergistic effects, Calcitriol combined with FK506 is more effective.
