CAJ | 학술논문

目的探讨高压氧治疗对严重烧伤大鼠肠黏膜屏障作用的影响。方法健康成年雄性SD大鼠45只，采用数字表法，随机分为假烫组(n=16)、烧伤组(n=14)和烧伤+HBO治疗组(n=15)。采用大鼠背部30％ TBSAⅢ度烫伤模型，高压氧治疗组在烧伤后24h始，在高压氧舱0.15 MPa( 1.5ATA)压力下，每日吸入95％以上浓度的氧气1h，连续5d。各组大鼠于伤后第7天取肠黏膜组织血及下腔静脉，观察各组肠黏膜组织病理变化差别，测血浆二胺氧化酶(DAO)、D-乳酸等指标。结果与假烫组相比，烧伤组肠黏膜出现明显病理改变，而应用高压氧治疗后，肠黏膜损伤明显减轻。烧伤后第7天大鼠血浆DAO和D-乳酸水平明显增高，而应用高压氧治疗后，上述两指标水平明显下降。结论高压氧治疗能够对严重烧伤大鼠肠黏膜屏障起到保护作用。
목적 탐토고압양치료대엄중소상대서장점막병장작용적영향。방법 건강성년웅성SD대서45지，채용수자표법，수궤분위가탕조(n=16)、소상조(n=14)화소상+HBO치료조(n=15)。채용대서배부30％ TBSAⅢ도탕상모형，고압양치료조재소상후24h시，재고압양창0.15 MPa( 1.5ATA)압력하，매일흡입95％이상농도적양기1h，련속5d。각조대서우상후제7천취장점막조직혈급하강정맥，관찰각조장점막조직병리변화차별，측혈장이알양화매(DAO)、D-유산등지표。결과 여가탕조상비，소상조장점막출현명현병리개변，이응용고압양치료후，장점막손상명현감경。소상후제7천대서혈장DAO화D-유산수평명현증고，이응용고압양치료후，상술량지표수평명현하강。결론 고압양치료능구대엄중소상대서장점막병장기도보호작용。
Objective To explore the effect of hyperbaric oxygen (HBO) on intestinal mucosa injury of severe burn rats. Methods Forty-five male healthy adult SD rats were randomly divided into 3 groups: the sham group(n = 16), the burn injury group(n = 14), and burn injury + HBO group(n = 15). The animals in burn injury groups sustained 3-degree burn injury on the back with a total body surface area (TBSA) of 30％.Twenty-four hours after bum injury, those in the HBO group were exposed to 95％ oxygen at a pressure of 0. 15MPa, 1 hour a day, for a succession of 5 days. On the 7th day after bum injury, blood samples from the intestinal mucosa and inferior vena cava were collected to detect differences in pathological changes in the intestinal mucosa of various groups, and serum levels of diamine oxidase (DAO) and D-lactate were also measured. Results When compared with the sham group, significant pathological changes in the intestinal mucosa could clearly be seen in the bum injury group. Following exposure to HBO, lesion in the intestinal mucosa improved obviously. On the 7th day after bum injury, levels of DAO and D-lactate increased significantly. However, after HBO treatment, the levels of DAO and D-lactate decreased significantly. Conclusions HBO seemed to have the effect of protecting the barrier of intestinal mucosa in severe burn rats.