CAJ | 학술논문

目的观察母体妊娠期间高叶酸(FA)饮食在子代小鼠先天性巨结肠(HD)发生中的作用.方法分别用0(低叶酸组)、2(正常对照组),40、160、320 mg FA/kg(高叶酸组)RHAA配方饲料补充孕小鼠,分别观察上述5组不同叶酸饮食环境下刚出生的仔鼠的结肠标本是否具有HD表型,并采取酶联免疫吸附试验(ELISA)检测孕鼠及仔鼠的血清叶酸浓度.结果随着饲料叶酸含量的升高,孕鼠及仔鼠血清叶酸浓度也呈相应逐渐升高,直至160 mg FA/kg饲料饮食,母鼠及仔鼠体内叶酸水平达到最高值；低叶酸组与正常对照组仔鼠无巨结肠发生；在高叶酸组中:40、160、320 mg3组巨结肠发生率分别为2.1％ (1/42)、6.1％ (2/33)、2.8％ (1/36),并与仔鼠血清叶酸浓度密切相关.结论母体妊娠期间高叶酸饮食可以诱导仔鼠HD的发生,高叶酸饮食因素在HD发病机制中有着重要作用.
목적 관찰모체임신기간고협산(FA)음식재자대소서선천성거결장(HD)발생중적작용.방법 분별용0(저협산조)、2(정상대조조),40、160、320 mg FA/kg(고협산조)RHAA배방사료보충잉소서,분별관찰상술5조불동협산음식배경하강출생적자서적결장표본시부구유HD표형,병채취매련면역흡부시험(ELISA)검측잉서급자서적혈청협산농도.결과 수착사료협산함량적승고,잉서급자서혈청협산농도야정상응축점승고,직지160 mg FA/kg사료음식,모서급자서체내협산수평체도최고치；저협산조여정상대조조자서무거결장발생；재고협산조중:40、160、320 mg3조거결장발생솔분별위2.1％ (1/42)、6.1％ (2/33)、2.8％ (1/36),병여자서혈청협산농도밀절상관.결론 모체임신기간고협산음식가이유도자서HD적발생,고협산음식인소재HD발병궤제중유착중요작용.
Objective To evaluate the role of high-dose folic acid (FA) diet in the pathogenesis of congenital magacolon in a mouse model during pregnancy.Methods One group was supplemented with low-dose FA (0 mg FA/kg diet),3 groups were treated with high-dose FA supplentment (40,160 and 320 mg FA/kg diet),and the last goup was given normal dosage FA diet (2 mg FA/kg diet).The blood serum FA concentration in pregnant female mice and filial generation mice were determined by using enzyme linked immunosorbent assay (ELISA).Results As FA supplements increased,blood serum FA concentration of mother and filial generation mice was increased correspondingly.The 160 mg FA/kg diet-treated group had the highest blood serum concentration of mother and filial generation mice.Megacolon phenotype was only detected in high-FA dosage groups (40,160 and 320 mg FA/kg diet),with the positive incidence being 1/42 (2.4％),2/33 (6.1％ ) and 1/36 (2.8％) respectively.Conclusion High-dose FA can induce congenital magacolon of mice at the duration of pregnancy.High-dose FA diet may play an important role in the development of Hirchsprung' s disease.