CAJ | 학술논문

目的研究阿魏酸钠(SF)对谷氨酸单钠(MSG)诱导的成年小鼠神经兴奋性毒性的保护作用.方法 60只昆明小鼠采用数字随机表法分为对照组、SF组、MSG组、MSG+SF组[根据SF剂量不同,又分为20、40、80mg/(kg·d)三个亚组],每组各10只.MSG组小鼠灌胃MSG[2.0g/(kg·d)],MSG+SF组MSG[2.0g/(kg·d)]灌胃的同时腹腔注射SF[20、40、80mg/(kg·d)],SF组腹腔注射SF[40mg/(kg·d)],每日1次,连续给药10d;对照组灌胃和腹腔注射同量生理盐水.通过小鼠的行为学实验和脑海马组织病理检查,分析MSG所引起的功能和形态学上的变化以及SF的保护作用.结果末次给药后第6天MSG组Y-迷宫正确反应次数比例(13.80/20)显著少于对照组(16.42/20),差异有统计学意义(P<0.01);MSG[2.0g/(kg·d)]+SF[(40mg/(kg·d)]组Y-迷宫正确反应次数比例(16.30/20)与对照组差异无统计学意义(P>0.05).MSG组小鼠海马细胞水肿、神经元变性坏死和组织增生;MSG[2.0g/(kg·d)]+SF[(40mg/(kg·d)]组海马未发现明显的组织病理学改变. 结论SF能部分抵消MSG诱导的成年小鼠行为障碍和脑海马病理组织损伤;SF对MSG诱导的成年小鼠神经兴奋性毒性存在保护作用.
목적 연구아위산납(SF)대곡안산단납(MSG)유도적성년소서신경흥강성독성적보호작용.방법 60지곤명소서채용수자수궤표법분위대조조、SF조、MSG조、MSG+SF조[근거SF제량불동,우분위20、40、80mg/(kg·d)삼개아조],매조각10지.MSG조소서관위MSG[2.0g/(kg·d)],MSG+SF조MSG[2.0g/(kg·d)]관위적동시복강주사SF[20、40、80mg/(kg·d)],SF조복강주사SF[40mg/(kg·d)],매일1차,련속급약10d;대조조관위화복강주사동량생리염수.통과소서적행위학실험화뇌해마조직병리검사,분석MSG소인기적공능화형태학상적변화이급SF적보호작용.결과 말차급약후제6천MSG조Y-미궁정학반응차수비례(13.80/20)현저소우대조조(16.42/20),차이유통계학의의(P<0.01);MSG[2.0g/(kg·d)]+SF[(40mg/(kg·d)]조Y-미궁정학반응차수비례(16.30/20)여대조조차이무통계학의의(P>0.05).MSG조소서해마세포수종、신경원변성배사화조직증생;MSG[2.0g/(kg·d)]+SF[(40mg/(kg·d)]조해마미발현명현적조직병이학개변. 결론SF능부분저소MSG유도적성년소서행위장애화뇌해마병리조직손상;SF대MSG유도적성년소서신경흥강성독성존재보호작용.
Objective To investigate a possible protective effect of sodium ferulate (SF) on monosodium glutamate (MSG)-induced neurotoxicity in adult mice. Methods Sixty mice were randomly divided into control, SF, MSG, and MSG+SF [20,40,80mg/(kg·d)] groups, n=10. The animals in MSG group received intragastric (ig) administration of MSG (2.0g/(kg·d)], the animals in the MSG+SF groups received simultaneously ig administration of MSG [2.0 g/(kg·d)] and intraperitoneal (ip) administration of SF [20,40,80mg/(kg·d)], the animals in SF group received ip administration of SF [40mg/ (kg·d)], and the animals in control group received ig and ip administration of normal saline, respectively, once-daily for 10d. On day 1 after the last ig administration of MSG or (and) SF the behavioural tests (test of Y-maze discrimination learning and open field test) were performed, and on day 4 after the treatment of MSG or (and) SF the histopathology of the animal brains was studied to analyze the MSG-induced functional and morphological changes and the possible protective effect of SF. Results The correct responses of Y-maze test on day 6 after the last administration of MSG and/or SF in MSG-treated group (13.83/20) were significantly less than those in control (16.42/20)(P<0.01), and those in MSG[2.0g/(kg·d)]+SF[40mg/(kg·d)]-treated mice (16.30/20) were close to those in control (P>0.05). Examination of histopathology displayed MSG-treated hippocampal lesions characterized by intracellular edema, degeneration and necrosis of neurons, and hyperplasia, and the hippocampal lesion did not appear in the MSG [2.0g/(kg·d)]+SF[40mg/(kg·d)]-treated mice. Conclusions SF partially countered the behavior disorders and hippocampal lesions induced by MSG; therefor, SF has a potent neuroprotection against MSG-induced neurotoxicity in adult mice.