CAJ | 학술논문

目的寻找肿瘤性胆囊息肉样病变(PLG)患者血清中的特异性蛋白,并建立诊断模型.方法应用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS)和WCX型磁珠检测23例肿瘤性PLG、21例非肿瘤性PLG和26例正常人的血清蛋白质指纹图谱,并联合应用Biomarker wizard及Biomarker Patterns Software软件分析处理数据.结果初步筛选出对诊断肿瘤性PLG有代表性的22个特异性蛋白,分析系统在设定条件下筛选出3个特异性蛋白(5064.52 Da,7916.33 Da,7643.32 Da)用于建立肿瘤性PLG的诊断模型.此模型对肿瘤性胆囊息肉样病变诊断的敏感度为100%,特异度为89.4%.结论 SELDI-TOF-MS技术可筛选出肿瘤性PLG的特异性蛋白并建立起肿瘤性PLG的诊断模型.
목적 심조종류성담낭식육양병변(PLG)환자혈청중적특이성단백,병건립진단모형.방법 응용표면증강격광해흡전리비행시간질보기술(SELDI-TOF-MS)화WCX형자주검측23례종류성PLG、21례비종류성PLG화26례정상인적혈청단백질지문도보,병연합응용Biomarker wizard급Biomarker Patterns Software연건분석처리수거.결과 초보사선출대진단종류성PLG유대표성적22개특이성단백,분석계통재설정조건하사선출3개특이성단백(5064.52 Da,7916.33 Da,7643.32 Da)용우건립종류성PLG적진단모형.차모형대종류성담낭식육양병변진단적민감도위100%,특이도위89.4%.결론 SELDI-TOF-MS기술가사선출종류성PLG적특이성단백병건립기종류성PLG적진단모형.
Objective To detect the serum specific proteins in tumor-like polypoid lesions of the gallbladder patients and establish diagnostic model.Methods Surface enhanced laser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS)technique and WCX Magnetic Beads were used to detect the serum Droteomic patterns of 23 patients with tumor-like PLG,21 patients with non tumor-like PLG and 26 normal persons.Biomarker Wizard and Biomarker Patterns Software were used in combination to analyze the data.Resuits Preliminary screening out 22 representative specific proteins for the diagnosis of the tumor like PLG.Analysis system under the conditions set selected 3 specific proteins to establish diagnostic model for the tumor-like PLG.The sensitivity and specificity of the model for the diagnosis of the tumor-like PLG were 100%and 89.4%,respectively.Conclusion SELDI-TOF-MS technique can select specific protein of the tumor-like PLG,and establish diagnostic model of the tumor-like PLG.