CAJ | 학술논문

目的:研究肥胖、2型糖尿病、胰岛素抵抗与β3-AR基因之间的关系及其在家系遗传中的规律.方法:运用PCR-RFLP技术分析了8个肥胖伴糖尿病家系的β3-AR基因外显子64位色氨酸/精氨酸(tryptophan to arginineTrp64Arg)多态性.结果:β3-AR基因与肥胖进行连锁,在常染色体显性遗传模式下,单个家系(家系3)最大LOD值为3.885 109(θ=20.000 00).将β3-AR基因与2型糖尿病进行连锁,在常染色体显性遗传模式下,单个家系(家系2)LOD最大值为0.222 336(θ=0.000 000),在常染色体隐性遗传模式下,单个家系(家系4)LOD最大值为0.805 003(θ=0.000 0000).结论:Trp64Arg多态性改变是部分家系肥胖产生的直接原因.
목적:연구비반、2형당뇨병、이도소저항여β3-AR기인지간적관계급기재가계유전중적규률.방법:운용PCR-RFLP기술분석료8개비반반당뇨병가계적β3-AR기인외현자64위색안산/정안산(tryptophan to arginineTrp64Arg)다태성.결과:β3-AR기인여비반진행련쇄,재상염색체현성유전모식하,단개가계(가계3)최대LOD치위3.885 109(θ=20.000 00).장β3-AR기인여2형당뇨병진행련쇄,재상염색체현성유전모식하,단개가계(가계2)LOD최대치위0.222 336(θ=0.000 000),재상염색체은성유전모식하,단개가계(가계4)LOD최대치위0.805 003(θ=0.000 0000).결론:Trp64Arg다태성개변시부분가계비반산생적직접원인.
Objective: To explore the association between the polymorphism of the β3-adrenergic receptor(R-AR) gene and obesity, type 2 diabetes, insulin resistance in Chinese pedigrees. Methods: Eight pedigrees with obesity and type 2 diabetes have been detected with the technique of PCR-RFLP in codon 64 of the exon region representing the variation Trp64 Arg of β3-AR gene. Results: In pedigree linkage analysis, the maximal LOD score of β3-AR gene with obesity was 3. 385 109 ( 0 = 0.000 000) at the mode of autosomal dominant in pedigree 3. The maximal LOD score of β3-AR with type 2 diabetes was 0.222336(0 =0. 000 000) at the mode of autosomal dominant in pedigree 2 and 0.805 003 ( 0 = 0.000 000) at the mode of autosomal recessive in pedigree 4.ial obesity. Pedigree linkage analysis can powerfully help to understand the action mechanism of the candidate genes. 2Better comphrehension the workings of adrenergic receptors should provide a new understanding of obesity, type 2 diabetes and insulin resistance and perhaps lead to new methods of treatment.