CAJ | 학술논문

背景:人发角蛋白人工腱(human hair keratin artificial tendon,HHKAT)材料是国内研制的新型生物可降解材料.目的:研究HHKAT材料在体内的降解及其对周围组织的影响.设计:完全随机双盲实验对照研究.地点和对象:在解放军昆明总医院动物实验基地,选用日本大耳白兔(普通级)12只,体质量(2.5±0.5)kg,雄性,标准饲料单笼喂养.干预:将12只兔随机分组,在脊旁肌埋藏处理30 min和处理15 min的人发角蛋白人工腱试件,用正常人发作对照.分别在2,6,12,24周取材.主要观察指标:对不同时期的材料进行大体及组织学观察.结果:实验中发现不同程度处理的HHKAT其降解速度不同,其中降解最快的处理30min组在24周时已完全吸收,而处理15 min组在24周时只有部分降解,对照组未见降解.HHKAT及人发在肌肉组织内无明显的炎症排斥反应,随着HHKAT材料的降解吸收,其周围的组织反应逐渐降低.结论:研究表明人发角蛋白人工腱材料在体内能够被降解吸收,可根据不同的需要调节其降解速度,具有良好的生物相容性.
배경:인발각단백인공건(human hair keratin artificial tendon,HHKAT)재료시국내연제적신형생물가강해재료.목적:연구HHKAT재료재체내적강해급기대주위조직적영향.설계:완전수궤쌍맹실험대조연구.지점화대상:재해방군곤명총의원동물실험기지,선용일본대이백토(보통급)12지,체질량(2.5±0.5)kg,웅성,표준사료단롱위양.간예:장12지토수궤분조,재척방기매장처리30 min화처리15 min적인발각단백인공건시건,용정상인발작대조.분별재2,6,12,24주취재.주요관찰지표:대불동시기적재료진행대체급조직학관찰.결과:실험중발현불동정도처리적HHKAT기강해속도불동,기중강해최쾌적처리30min조재24주시이완전흡수,이처리15 min조재24주시지유부분강해,대조조미견강해.HHKAT급인발재기육조직내무명현적염증배척반응,수착HHKAT재료적강해흡수,기주위적조직반응축점강저.결론:연구표명인발각단백인공건재료재체내능구피강해흡수,가근거불동적수요조절기강해속도,구유량호적생물상용성.
BACKGROUND: Human hair keratin artificial tendon (HHKAT) material is a new biological degradable material established in China.OBJECTIVE: To investigate the degradation of HHKAT material in vivo as well as its impact on surrounding tissues.DESIGN: A completely randomized, double-blind controlled trial was conducted.SETTING and PARTICIPANTS: The study was conducted in the Center of Experimental Animals, Kunming General Hospital of Chengdu Military Area Command of Chinese PLA. Twelve male Japanese rabbits(ordinary grade)with a body mass of (2.5 ±0. 5)kg were selected. The animals were fed with standard feed in separate cage.INTERVENTIONS: The rabbits were randomly grouped. HHKAT samples with a disposal time of 30 minutes and 15 minutes were embedded respectively in paraspinal muscle. Normal human hair was used as control group.Materials were accessed respectively at 2, 6, 12 and 24 weeks.MAIN OUTCOME MEASURES: Gross and histological observation of the material was performed in different periods.RESULTS: The degradation speed was different in HHKAT of different disposal time. The 30-minute group had the fastest degradation, which had been absorbed completely in 24 weeks; however, the 15-minute group had only partial degradation in 24 weeks; and control group had no degradation.Histological investigation showed that HHKAT and normal human hair do not evoke obvious inflammatory reactions, and the tissue reaction around gradually decreased with the degradation and absorption of HHKAT.CONCLUSION: HHKAT is a degradable and absorbable material with good biocompatibility, which the degradation speed can be adjusted according to different requirements.