CAJ | 학술논문

采用pH区带精制逆流色谱法从广西地不容中制备青风藤碱，溶剂系统为石油醚-乙酸乙酯-甲醇-水（3：7：1：9，V／V），上相加10mmol／L三乙胺为固定相，下相加10mmol／L盐酸为流动相。一次进样1．0g，可得纯度为99．5％的青风藤碱600mg，所得产物经ESI-MS，1H NMR和13C NMR进行结构鉴定。本方法效率高，制品纯度高，适合青风藤碱的大量制备。
채용pH구대정제역류색보법종엄서지불용중제비청풍등감，용제계통위석유미-을산을지-갑순-수（3：7：1：9，V／V），상상가10mmol／L삼을알위고정상，하상가10mmol／L염산위류동상。일차진양1．0g，가득순도위99．5％적청풍등감600mg，소득산물경ESI-MS，1H NMR화13C NMR진행결구감정。본방법효솔고，제품순도고，괄합청풍등감적대량제비。
We employed pH-zone refining counter-current chromatography to prepare high-purity sinoacutine from Stephania kwangsiensis. Its solvent system was composed of petroleum ether-ethyl acetate-methanol-water （3 ： 7： 1 ： 9, V/V）, in which triethylamine （ 10 mmol/L） was added to the upper organic stationary phase as a retainer and hydrochloric acid （10 mmol/L） was added to the aqueous mobile phase as an eluter. The structure of sinoautine was identified by ESI-MS,1H NMR and 13C NMR. The purity of sinoacutine determined by RP-HPLC was 99.5%. This approach has such advantages as high-efficiency and high-purity, suitable for the preparation of large amount of sinoacutine.