CAJ | 학술논문

目的探讨黄芩苷对抗结核药物肝损伤的保护作用机制.方法将60只小鼠完全随机分为6组,即正常对照组,肝损伤 (异烟肼+利福霉素钠) 组,联苯双酯组及黄芩苷高、中、低剂量组.给药8 d后,分别用光镜和电镜观察肝脏组织细胞病理学的改变情况,用免疫组织化学染色技术分析肿瘤坏死因子-α(TNF-α)的表达情况.结果黄芩苷能明显减轻肝细胞变性和坏死,炎症活动程度明显减轻(P<0.05 或P<0.01);TNF-α蛋白在黄芩苷组小鼠肝脏组织内为弱阳性表达(P<0.05 或P<0.01);黄芩苷3个剂量组间小鼠肝脏病理形态学及肝脏组织内TNF-α蛋白的表达均无显著性差异(P>0.05).结论黄芩苷对抗结核药物肝损伤的保护作用可能与抑制TNF-α蛋白表达有关.
목적 탐토황금감대항결핵약물간손상적보호작용궤제.방법 장60지소서완전수궤분위6조,즉정상대조조,간손상 (이연정+리복매소납) 조,련분쌍지조급황금감고、중、저제량조.급약8 d후,분별용광경화전경관찰간장조직세포병이학적개변정황,용면역조직화학염색기술분석종류배사인자-α(TNF-α)적표체정황.결과 황금감능명현감경간세포변성화배사,염증활동정도명현감경(P<0.05 혹P<0.01);TNF-α단백재황금감조소서간장조직내위약양성표체(P<0.05 혹P<0.01);황금감3개제량조간소서간장병리형태학급간장조직내TNF-α단백적표체균무현저성차이(P>0.05).결론 황금감대항결핵약물간손상적보호작용가능여억제TNF-α단백표체유관.
Objective To investigate the protective effects of baicalin on the hepatic injury induced by antituberculosis drugs in mice.Methods Sixty mice were divided randomly into 6 groups:normal control group,liver injury isoniazid and rifamycin sodium group,positive control group,baicalin groups(high,middle and low doses).The drugs were administered to mice once daily for 8 days.Light microscope and electronic microscope were used respectively to observe the histopathological changes of the hepatic cells.The expression of tumor necrosis factor-α (TNF-α) was detected by immunohistochemistry.Results Baicalin alleviated the degeneration and necrosis induced by antituberculosis drugs obviously.The level of inflammatory activity was relieved significantly (P<0.05,P<0.01).The immunoreactivity of TNF-α protein in liver cells was slightly stained in baicalin groups (P<0.05,P<0.01).There were no significant differences about the pathomorphology,the inflammatory activity and the express of TNF-α protein among baicalin groups (P>0.05).Conclusion The protective effects of baicalin on hepatic injury induced by antituberculosis drugs may be related to inhibit TNF-α protein expression.