CAJ | 학술논문

对8头健康和8头患子宫内膜炎奶牛经子宫内灌注土霉素注射液,研究了土霉素在奶牛血浆中的药动学特征.应用反向高效液相色谱法为检测手段对血浆中土霉素浓度进行测定,采用3p97药动学软件分析药-时数据.结果表明:土霉素在健康奶牛子宫内不易被吸收入血,而在患病奶牛血浆中的最高血药浓度较低.土霉素在患子宫内膜炎奶牛体内的药动学特征符合一级吸收一室开放模型,主要药动学参数:药时曲线下面积(AUC)为(18.53±10.92) mg·L~(-1)·h~(-1),血药峰浓度(C_(max))为(1.66±0.84)μg/mL,达峰时间(t_(max))为(0.35 ±0.06) h,吸收半衰期(t_(1/2ka))为(0.05±0.01) h,清除半衰期(t_(1/2ke))为(7.67±2.44) h.土霉素灌注给药在患子宫内膜炎的奶牛子宫内吸收快但不完全,消除较慢.
대8두건강화8두환자궁내막염내우경자궁내관주토매소주사액,연구료토매소재내우혈장중적약동학특정.응용반향고효액상색보법위검측수단대혈장중토매소농도진행측정,채용3p97약동학연건분석약-시수거.결과표명:토매소재건강내우자궁내불역피흡수입혈,이재환병내우혈장중적최고혈약농도교저.토매소재환자궁내막염내우체내적약동학특정부합일급흡수일실개방모형,주요약동학삼수:약시곡선하면적(AUC)위(18.53±10.92) mg·L~(-1)·h~(-1),혈약봉농도(C_(max))위(1.66±0.84)μg/mL,체봉시간(t_(max))위(0.35 ±0.06) h,흡수반쇠기(t_(1/2ka))위(0.05±0.01) h,청제반쇠기(t_(1/2ke))위(7.67±2.44) h.토매소관주급약재환자궁내막염적내우자궁내흡수쾌단불완전,소제교만.
The plasma pharmacokinetics of oxytetracycline (OTC) in 8 healthy and 8 endometritis affected cows was evaluated by using HPLC as a quantitative method.The data for the plasma concentrations of OTC were analysed using 3p97 software package.Results showed that there were no detectable concentrations in healthy cows,while the maximum concentration in cows affected by endometritis was relatively low.A one compartment open model with first order absorption provided the best fit for all the individual data based on the Akaike information criterion.The main pharmacokinetic parameters were as follow:AUC (18.53±10.92) mg·L~(-1)·h~(-1),C_(max) (1.66±0.84) μg/mL,t_(max) (0.35±0.06) h,t_(1/2ka) (0.05±0.01) h and t_(1/2ke) (7.67±2.44)h,respectively.Results showed that OTC was poorly absorbed with a fast absorption rate,and it was eliminated slowly from plasma after single intrauterine administration.