应用化学
應用化學
응용화학
CHINESE JOURNAL OF APPLIED CHEMISTRY
2010年
2期
246-248
,共3页
加压毛细管电色谱%甲基苯基丙胺%对映体拆分%羟丙基-β-环糊精
加壓毛細管電色譜%甲基苯基丙胺%對映體拆分%羥丙基-β-環糊精
가압모세관전색보%갑기분기병알%대영체탁분%간병기-β-배호정
pressurized capillary electrochromatography(pCEC)%dimethylphenylpropylamine%enantiomer separation%hydroxypropyl-β-cyclodextrin
运用毛细管电色谱(pCEC)模式,以羟丙基-β-环糊精(HP-β-CD)作为手性选择剂,对1-甲基-3-苯基丙胺对映体进行手性分离.1-甲基-3-苯基丙胺对映体在最佳的条件下如:手性选择剂浓度10 g/L、流动相配比:V(乙腈):V(磷酸盐体系)=60:40溶液(5 mmol/L)、背景电解质pH=7.6、柱温16 ℃和分离电压10 kV达到了基线分离,该方法重现性好、简便、快捷.
運用毛細管電色譜(pCEC)模式,以羥丙基-β-環糊精(HP-β-CD)作為手性選擇劑,對1-甲基-3-苯基丙胺對映體進行手性分離.1-甲基-3-苯基丙胺對映體在最佳的條件下如:手性選擇劑濃度10 g/L、流動相配比:V(乙腈):V(燐痠鹽體繫)=60:40溶液(5 mmol/L)、揹景電解質pH=7.6、柱溫16 ℃和分離電壓10 kV達到瞭基線分離,該方法重現性好、簡便、快捷.
운용모세관전색보(pCEC)모식,이간병기-β-배호정(HP-β-CD)작위수성선택제,대1-갑기-3-분기병알대영체진행수성분리.1-갑기-3-분기병알대영체재최가적조건하여:수성선택제농도10 g/L、류동상배비:V(을정):V(린산염체계)=60:40용액(5 mmol/L)、배경전해질pH=7.6、주온16 ℃화분리전압10 kV체도료기선분리,해방법중현성호、간편、쾌첩.
1-Dimethyl-3-phenylpropylamine enantiomer was separated with hydroxypropyl-β-cyclodextrin(HP-β-CD) as chiral selector by means of pressurized capillary electrochromatography(pCEC). The optimal experimental conditions are HP-β-CD concentration 10 g/L, background buffer pH=7.6, capillary temperature 16 ℃, voltage 10 kV, and the mobile phase consisted of a solution of acetonitrile and phosphate at a volume ratio of V(CH_3CN):V(NaH_2PO_4-Na_2HPO_4)=60:40. The method is simple, rapid and reproducible.
