西南师范大学学报(自然科学版)
西南師範大學學報(自然科學版)
서남사범대학학보(자연과학판)
JOURNAL OF SOUTHWEST CHINA NORMAL UNIVERSITY
2010年
1期
119-123
,共5页
孙雪梅%乔爱民%孙敏%桂腾琴%尹彩霞
孫雪梅%喬愛民%孫敏%桂騰琴%尹綵霞
손설매%교애민%손민%계등금%윤채하
菜心%遗传多样性%ISSR
菜心%遺傳多樣性%ISSR
채심%유전다양성%ISSR
Brassica campestris L. ssp. chinensis var. utilis Tssen. et Lee.%genetic diversity%ISSR
利用ISSR技术对27个菜心品种的遗传多样性进行了分析.结果表明,12条引物共检测到103个可重复位点,其中多态位点58个,多态位点百分率(P)为56.31%.平均多样性指数(1)为0.229,遗传相似系数(H)为0.144,观察等位基因数(Na)和有效等位基因数(Ne)分别为1.563和1.225,遗传距离(GD)的范围在0.029~0.344之间,表明菜心遗传多样性较低.经UPGMA聚类分析,将27个菜心品种分为六大类群.
利用ISSR技術對27箇菜心品種的遺傳多樣性進行瞭分析.結果錶明,12條引物共檢測到103箇可重複位點,其中多態位點58箇,多態位點百分率(P)為56.31%.平均多樣性指數(1)為0.229,遺傳相似繫數(H)為0.144,觀察等位基因數(Na)和有效等位基因數(Ne)分彆為1.563和1.225,遺傳距離(GD)的範圍在0.029~0.344之間,錶明菜心遺傳多樣性較低.經UPGMA聚類分析,將27箇菜心品種分為六大類群.
이용ISSR기술대27개채심품충적유전다양성진행료분석.결과표명,12조인물공검측도103개가중복위점,기중다태위점58개,다태위점백분솔(P)위56.31%.평균다양성지수(1)위0.229,유전상사계수(H)위0.144,관찰등위기인수(Na)화유효등위기인수(Ne)분별위1.563화1.225,유전거리(GD)적범위재0.029~0.344지간,표명채심유전다양성교저.경UPGMA취류분석,장27개채심품충분위륙대류군.
Genetic diversity of 27 flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tssen. et Lee) cultivars were analyzed with inter--simple sequence repeat (ISSR) molecular mark-ers. A total of 103 loci were detected using 12 ISSR primers, of which 58 were polymorphic, the percent-age of polymorphic loci (p) being 56.31%. Shannon's information index (I) and Nei's gene diversity (H) were 0.229 and 0.144, respectively, observed number of alleles (Na) and effective number of alleles (Ne) were 1.563 and 1.225, respectively, and the genetic distance ranged from 0.029 to 0.344, indicating that the genetic diversity of flowering Chinese cabbage was relatively low. UPGMA cluster analysis clustered the 27 cultivars into six groups.