中国组织化学与细胞化学杂志
中國組織化學與細胞化學雜誌
중국조직화학여세포화학잡지
CHINESE JOURNAL OF HISTOCHEMISY AND CYTOCHEMISY
2010年
1期
26-31
,共6页
吴靖芳%顾彩霞%张静%郑慧娥%王浩宇%张江兰
吳靖芳%顧綵霞%張靜%鄭慧娥%王浩宇%張江蘭
오정방%고채하%장정%정혜아%왕호우%장강란
肠三叶因子%胃溃疡%下颌下腺%大鼠%免疫组织化学%RT-PCR
腸三葉因子%胃潰瘍%下頜下腺%大鼠%免疫組織化學%RT-PCR
장삼협인자%위궤양%하합하선%대서%면역조직화학%RT-PCR
Trefoil factor3%Gastric ulcer%Submaxilary gland%Rat%Immunhistochemistry%RT-PCR
目的 研究下颌下腺肠三叶因子(intestinal trefoil peptide,ITF即TFF3)基因在大鼠实验性胃溃疡自愈过程的变化,探讨其与胃溃疡自愈的关系.方法 通过胃窦前壁黏膜下注射冰乙酸制备大鼠胃溃疡模型:⑴用免疫组织化学SABC法和RT-PCR检测 42只溃疡组,21只盐水组,及6只正常组大鼠下颌下腺组织中TFF3肽和TFF3mRNA的表达情况.结果 (1)免疫组化显示:溃疡组大鼠下颌下腺的TFF3肽主要表达于导管系统上皮,如闰管、颗粒曲管(granular convoluted tubule,GCT)以及纹状管、小叶间导管上皮细胞、黏液腺泡细胞也有少量分布,浆液腺泡细胞呈阴性.溃疡组手术后第1d时,下颌下腺TFF3表达明显强于盐水组和正常组(P<0.01).术后第2d,积分光密度明显低于1d溃疡组(P<0.05),4、6d积分光密度逐渐增强并高于对照组(P<0.05),到术后第10d达高峰(P<0.01),23d积分光密度仍高于对照组(P<0.05).(2)RT-PCR显示:溃疡1、2、4、6、10、14、23d TFF3/GAPDH光密度比值分别为1.42±0.10 ,1.18±0.13,1.29±0.15,1.24±0.17, 1.57±0.19, 1.25±0.14,1.13±0.16明显高于相应盐水对照组的TFF3/GAPDH光密度比值(P<0.01).结论 大鼠胃溃疡时期,下颌下腺TFF3基因上调,推测下颌下腺TFF3通过外分泌或内分泌参与胃溃疡愈合过程.
目的 研究下頜下腺腸三葉因子(intestinal trefoil peptide,ITF即TFF3)基因在大鼠實驗性胃潰瘍自愈過程的變化,探討其與胃潰瘍自愈的關繫.方法 通過胃竇前壁黏膜下註射冰乙痠製備大鼠胃潰瘍模型:⑴用免疫組織化學SABC法和RT-PCR檢測 42隻潰瘍組,21隻鹽水組,及6隻正常組大鼠下頜下腺組織中TFF3肽和TFF3mRNA的錶達情況.結果 (1)免疫組化顯示:潰瘍組大鼠下頜下腺的TFF3肽主要錶達于導管繫統上皮,如閏管、顆粒麯管(granular convoluted tubule,GCT)以及紋狀管、小葉間導管上皮細胞、黏液腺泡細胞也有少量分佈,漿液腺泡細胞呈陰性.潰瘍組手術後第1d時,下頜下腺TFF3錶達明顯彊于鹽水組和正常組(P<0.01).術後第2d,積分光密度明顯低于1d潰瘍組(P<0.05),4、6d積分光密度逐漸增彊併高于對照組(P<0.05),到術後第10d達高峰(P<0.01),23d積分光密度仍高于對照組(P<0.05).(2)RT-PCR顯示:潰瘍1、2、4、6、10、14、23d TFF3/GAPDH光密度比值分彆為1.42±0.10 ,1.18±0.13,1.29±0.15,1.24±0.17, 1.57±0.19, 1.25±0.14,1.13±0.16明顯高于相應鹽水對照組的TFF3/GAPDH光密度比值(P<0.01).結論 大鼠胃潰瘍時期,下頜下腺TFF3基因上調,推測下頜下腺TFF3通過外分泌或內分泌參與胃潰瘍愈閤過程.
목적 연구하합하선장삼협인자(intestinal trefoil peptide,ITF즉TFF3)기인재대서실험성위궤양자유과정적변화,탐토기여위궤양자유적관계.방법 통과위두전벽점막하주사빙을산제비대서위궤양모형:⑴용면역조직화학SABC법화RT-PCR검측 42지궤양조,21지염수조,급6지정상조대서하합하선조직중TFF3태화TFF3mRNA적표체정황.결과 (1)면역조화현시:궤양조대서하합하선적TFF3태주요표체우도관계통상피,여윤관、과립곡관(granular convoluted tubule,GCT)이급문상관、소협간도관상피세포、점액선포세포야유소량분포,장액선포세포정음성.궤양조수술후제1d시,하합하선TFF3표체명현강우염수조화정상조(P<0.01).술후제2d,적분광밀도명현저우1d궤양조(P<0.05),4、6d적분광밀도축점증강병고우대조조(P<0.05),도술후제10d체고봉(P<0.01),23d적분광밀도잉고우대조조(P<0.05).(2)RT-PCR현시:궤양1、2、4、6、10、14、23d TFF3/GAPDH광밀도비치분별위1.42±0.10 ,1.18±0.13,1.29±0.15,1.24±0.17, 1.57±0.19, 1.25±0.14,1.13±0.16명현고우상응염수대조조적TFF3/GAPDH광밀도비치(P<0.01).결론 대서위궤양시기,하합하선TFF3기인상조,추측하합하선TFF3통과외분비혹내분비삼여위궤양유합과정.
Objective To explore the expression of trefoil factor3 (TFF3) gene in submaxilary glands during the self-healing of experimental gastric ulcer in rats. Methods Gastic ulcer was induced by injecting acetic acid to the mucosa of paries anterior gastricus. The expressions of TFF3 peptide and TFF3mRNA in 42 rats with gastric ulcer, 21 control rats and 6 normal rats were detected respectively by immunohistochemical SABC method and RT-PCR. Results (1)TFF3 immunoreactive cells were mainly located in the epithelium cytoplasm of intercalated ducts, GCT,striated ducts, and interlobular ducts,and in the mucous acinus,but not in serous acinus.TFF3 expression was stronger in gastric ulcer than in the other two groups on the first day( P<0.01).The integral optical density (IOD) values decreased on the 2nd day when compared with that of the 1st day,then increased gradually on day 4 and 6,reached the peak on the 10th day(P<0.01), and kept a high level on the 23rd day compared with that of the corresponding control group (P<0.05). (2)The OD value of TFF3/GAPDH of the gastric ulcer group on 1,2,4,6,10,14,23d was 1.42±0.10,1.18±0.13,1.29±0.15,1.24±0.17, 1.57±0.19, 1.25±0.14,1.13±0.16,respectively.They were obviously higher than those of the saline control group(P<0.01).Conclusion The TFF3 gene is up-regulated in rat submaxilary glands,so we infer that TFF3 gene may participate in the regulation of self-healing in gastric ulcer.
