林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2010年
4期
151-155,封3
,共6页
徐华潮%徐金华%张立钦%林海萍%杨萍%程剑明
徐華潮%徐金華%張立欽%林海萍%楊萍%程劍明
서화조%서금화%장립흠%림해평%양평%정검명
苏云金杆菌%松墨天牛%生物防治%松材线虫病
囌雲金桿菌%鬆墨天牛%生物防治%鬆材線蟲病
소운금간균%송묵천우%생물방치%송재선충병
Bacillus thuringiensis%Monochamus alternatus%biological control%pine wilt disease
The virulence of 17 Bacillus thuringiensis strains against 2-3 instar larvae of Monochamus alternatus were tested in indoor.The results showed that two strains (RBT-200701, RBT-200702) exhibited high poisonous activity, and the caused corrected mortality of 2-3 instar M.alternatus larvae were all more than 70%.A positive correlation was found between the corrected mortality and the concentration of spores and parasporal crystal(125-2 000 μg·g-1). The insecticidal effect increased with treatment time (0-20 d).The morphological changes of the two strains at various developmental stages were examined with microscopy on the cell smear.The optimal fermentation condition of strains was 30 ℃, pH7.2,200 r·min-1,for 36 h. The two strains had the same protein spectrum after SDS-PAGE analysis.
