CAJ | 학술논문

目的探讨新疆反复呼吸道感染儿童(复感儿)血清甘露聚糖结合凝集素(MBL)水平与MBL第一外显子54密码子基因多态性的关系.方法用酶联免疫分析方法检测6个月至10岁复感儿73例(复感儿组)与同龄健康儿童92例(对照组)的血清MBL水平；用聚合酶链反应(PCR) -限制性内切酶片段长度多态性分析(RFLP)方法检测上述复感儿中73例的MBL第一外显子54密码子基因多态性以及突变频率.结果复感儿血清MBL水平为(2.2±1.2)ng/L,健康儿童血清MBL水平为(3.3±2.1)ng/L,2组差异有统计学意义(P＜0.05).复感儿血清MBL水平明显低于健康儿童.新疆复感儿MBL-54密码子突变频率为27.4％,结果与国内报道的健康儿童突变频率1.8％相比较,明显高于国内正常儿童(P＜0.05).复感儿MBL-54密码子突变的频率在维吾尔族(9％,7/22)和汉族(18％,13/51)间的差异无统计学意义；不同性别的复感儿MBL-54密码子突变的频率差异无统计学意义.结论新疆复感儿MBL-54密码子突变频率高于健康儿童,使得复感儿血清MBL水平较正常儿童低,造成免疫水平低下,是这些儿童表现反复呼吸道感染原因之一.
목적 탐토신강반복호흡도감염인동(복감인)혈청감로취당결합응집소(MBL)수평여MBL제일외현자54밀마자기인다태성적관계.방법 용매련면역분석방법검측6개월지10세복감인73례(복감인조)여동령건강인동92례(대조조)적혈청MBL수평；용취합매련반응(PCR) -한제성내절매편단장도다태성분석(RFLP)방법검측상술복감인중73례적MBL제일외현자54밀마자기인다태성이급돌변빈솔.결과 복감인혈청MBL수평위(2.2±1.2)ng/L,건강인동혈청MBL수평위(3.3±2.1)ng/L,2조차이유통계학의의(P＜0.05).복감인혈청MBL수평명현저우건강인동.신강복감인MBL-54밀마자돌변빈솔위27.4％,결과여국내보도적건강인동돌변빈솔1.8％상비교,명현고우국내정상인동(P＜0.05).복감인MBL-54밀마자돌변적빈솔재유오이족(9％,7/22)화한족(18％,13/51)간적차이무통계학의의；불동성별적복감인MBL-54밀마자돌변적빈솔차이무통계학의의.결론 신강복감인MBL-54밀마자돌변빈솔고우건강인동,사득복감인혈청MBL수평교정상인동저,조성면역수평저하,시저사인동표현반복호흡도감염원인지일.
Objective To investigate the relations between gene polymorphism at code 54 of exon 1 and serum protein level of mannose binding lectin(MBL) in children with recurrent respiratory tract infections (RRTI).Methods To detect the serum MBL level and observe the mutation on code 54 of exon 1 of MBL in 92 healthy children and 77 RRTI with enzyme-linked immunoabsordent assay and polymerasechain reaction( PCR)-restriction fragment length polymorphism (RFLP).Results The evaluation in healthy children and children with RRTI was (3.3 ±2.1)ng/L and (2.2 ± 1.2)ng/L,respectively.There was a significant difference (P ＜0.05).Serum MBL level in children with RRT were lower than that in healthy children.Frequencies at code 54 exon 1 of children with RRTI in xinjiang were 27.4％,which was higher in healthy children of home which was 1.8％.Frequencies at code 54 exon 1 did not show significant difference between Uyghur and Han nationalities.The mutation frequencies at the code 54 of exon 1 did not show significant difference between male and female.Conclusion The mutation on code 54 of exon 1 of MBL is the important cause of the low serum MBL level in children with RRTI.